The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to phy...The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to physical and chemical agents. Some plant extracts, such as soluble wheat extract, have been shown to be effective in inhibiting or destroying the biofilm of certain micro-organisms under specific conditions. The objective of this study is to evaluate the capacity of the pathogen to form biofilm on different surfaces used in livestock, as well as to evaluate the anti-biofilm capacity of the soluble wheat extract against <i>S. aureus</i> on these surfaces. The inhibition potential of inhibition or destruction of biofilm was tested in vitro. Wheat extract at a concentration of 0.29 mg/100mL showed anti-biofilm activity on <i>S. aureus</i>, inhibiting its formation, as well as destroying it greatly after a contact time of 24 hours, on those surfaces where the microorganism presents more adhesion capacity.展开更多
[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using m...[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using modified CTAB method. The extracted DNA was detected by 0.8% agarose gel electrophoresis. [ Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method, and was suitable for carrying out normal PCR amplification. [ Conclusion] This study provides a simple and quick method for extracting DNA from wheat with a spot of material.展开更多
[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prep...[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prepare the extracts from walnut green husk. The toxicity and antifeedant activity on wheat aphid were determined, [ Result ] Walnut green husk extracts in the solvent of ethanol, acetone, ethyl acetate had higher toxicity and antifeedant activity on wheat aphid. When the concentration was 100 mg/ml, after 24 h, the toxicity and antifeedant activity of three kinds extracts on wheat aphid were greater than 84.35% and 66.98%, respectively. After 48 h, the toxicity and antifeedant activity were greater than 85.91% and 79.34%, respectively. Among 5 kinds extracts,ethanol extracts had the highest toxicity and antifeedant activity on wheat aphid. After 24 h, the corrected mortality rate was 93.64% and the antifeedant rate was 83.61%. After 48 h,the corrected mortality rate was 94.95% and antifeedant rate was 92.21%. Petroleum ether extract had the smallest toxicity and antifeedant activity on wheat aphid. After 24 h,the corrected mortality rate and antifeedant rate were 19.54% and 34.65%, respectively. After 48 h,the corrected mortality rate and antifeedant rate were 20.13% and 39. 03%, respectively. [ Conclsion] The extracts from walnut green husk with larger polarity solvent had better toxicity and higher antifeedant activity on wheat aphid.展开更多
Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermen...Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 （LFWGE）. Methods The HT-29 cells were transplanted via subcutaneous injection of 1×10^7cells into the right flank of each nude mouse. Then, nude mice were treated for 30 d with LFWGE （high-dose 2 g/kg/d; low-dose 1 g/kg/d） and for 7 d with 5-fluorouracil （5-FU, 25 mg/kg/d） by gavage and intraperitoneal injection, respectively. An inhibition of tumor growth was observed. Results Tumor volume and weights decreased significantly in both groups of nude mice treated with LFWGE. In addition, the cell apoptosis rate of the LFWGE group （2 g/kg/d, 60.2%＋4.4%; 1 g/kg/d, 58.6%＋6.9%） was significantly higher than that of the control group （11.5%＋1.6%） and 5-FU group （32.1%＋3.5%） as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot method further confirmed these enhancing apoptosis and growth inhibition effects. The involvement of LFWGE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, Caspase-3, and CyclinD1. Conclusion The results showed that LFWGE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be as a natural nutrient supplements or chemopreventive agent in the treatment of human colon cancer.展开更多
We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma...We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC;this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a facile way of improving the overall sensitivity with which these foreign proteins can be detected in equine plasma.展开更多
Wheat blast, caused by a fungal pathogen, <i><span style="font-family:Verdana;">Magnaporthe oryzae Tritichum </span></i><span style="font-family:Verdana;">(</span&g...Wheat blast, caused by a fungal pathogen, <i><span style="font-family:Verdana;">Magnaporthe oryzae Tritichum </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">MoT</span></i><span style="font-family:Verdana;">) pathotype (wheat isolates), was first reported in Bangladesh among Asian countries in 2016. It is a serious disease of wheat causing yield failures and significant economic losses during epidemic years. Extensive use of persistent synthetic pesticides to control notorious wheat blast imposes enormous threat to human health and environment. In addition, the increasing demand for organic food has stimulated people to look for alternative methods. Nowadays, the need for synthetic chemical-free agricultural practices is gaining importance due to effective in managing crop pests, inexpensive, biodegradable, easily available and have low toxicity to non-target organisms. In order to identify active plants, 12 kinds of methanol extracts obtained from 12 medicinal plants were conducted </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to test the effect against </span><i><span style="font-family:Verdana;">Magnaporthe oryzae Triticum </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">MoT</span></i><span style="font-family:Verdana;">) pathotype. The remarkable inhibitory activity {(29.6 ± 01.5) mm, (25.1 ± 01.0) mm and (20.0 ± 02.0) mm zone of inhibition)} exhibited by the extracts (5 mg/disk) obtained from </span><i><span style="font-family:Verdana;">Artemisia indica</span></i><span style="font-family:Verdana;"> (Nagdona), </span><i><span style="font-family:Verdana;">Persicaria orientalis</span></i><span style="font-family:Verdana;"> (Bishkatali) and </span><i><span style="font-family:Verdana;">Clerodendrum indicum</span></i><span style="font-family:Verdana;"> (Bamonhati), respectively. Other medicinal plant extracts did not show any significant or no activity at all. Therefore, the three plant extracts might be a promising source for developing natural fungicides against wheat blast.</span>展开更多
文摘The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to physical and chemical agents. Some plant extracts, such as soluble wheat extract, have been shown to be effective in inhibiting or destroying the biofilm of certain micro-organisms under specific conditions. The objective of this study is to evaluate the capacity of the pathogen to form biofilm on different surfaces used in livestock, as well as to evaluate the anti-biofilm capacity of the soluble wheat extract against <i>S. aureus</i> on these surfaces. The inhibition potential of inhibition or destruction of biofilm was tested in vitro. Wheat extract at a concentration of 0.29 mg/100mL showed anti-biofilm activity on <i>S. aureus</i>, inhibiting its formation, as well as destroying it greatly after a contact time of 24 hours, on those surfaces where the microorganism presents more adhesion capacity.
基金Supported by National GMO Cultivation of New Varieties of Major Projects "Anti-reverse to Cultivate New Varieties of Genetically Modified Wheat," a Major IssueNational Science and Technology Support Program Topics (2006BAD01A02-8)National System of Industrial Science and Technology of Modern Wheat Comprehensive Experimental Station in Shanxi Province and National public Service Sector(Agriculture) Research Project (Shanxi Province)(nycytx-03)~~
文摘[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using modified CTAB method. The extracted DNA was detected by 0.8% agarose gel electrophoresis. [ Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method, and was suitable for carrying out normal PCR amplification. [ Conclusion] This study provides a simple and quick method for extracting DNA from wheat with a spot of material.
基金Supported by Natural Science Foundation of Ningxia Hui Autonomous Region(NZ09205)~~
文摘[Objective] The research aimed to study the toxicity and antifeedant activity of walnut green husk extract on wheat aphid. [Method] Using ultrasonic extraction, 5 kinds of different polarity solvents were used to prepare the extracts from walnut green husk. The toxicity and antifeedant activity on wheat aphid were determined, [ Result ] Walnut green husk extracts in the solvent of ethanol, acetone, ethyl acetate had higher toxicity and antifeedant activity on wheat aphid. When the concentration was 100 mg/ml, after 24 h, the toxicity and antifeedant activity of three kinds extracts on wheat aphid were greater than 84.35% and 66.98%, respectively. After 48 h, the toxicity and antifeedant activity were greater than 85.91% and 79.34%, respectively. Among 5 kinds extracts,ethanol extracts had the highest toxicity and antifeedant activity on wheat aphid. After 24 h, the corrected mortality rate was 93.64% and the antifeedant rate was 83.61%. After 48 h,the corrected mortality rate was 94.95% and antifeedant rate was 92.21%. Petroleum ether extract had the smallest toxicity and antifeedant activity on wheat aphid. After 24 h,the corrected mortality rate and antifeedant rate were 19.54% and 34.65%, respectively. After 48 h,the corrected mortality rate and antifeedant rate were 20.13% and 39. 03%, respectively. [ Conclsion] The extracts from walnut green husk with larger polarity solvent had better toxicity and higher antifeedant activity on wheat aphid.
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Industry-academic Joint Technological Innovations Funded Project of Jiangsu Province(BY2012172)
文摘Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 （LFWGE）. Methods The HT-29 cells were transplanted via subcutaneous injection of 1×10^7cells into the right flank of each nude mouse. Then, nude mice were treated for 30 d with LFWGE （high-dose 2 g/kg/d; low-dose 1 g/kg/d） and for 7 d with 5-fluorouracil （5-FU, 25 mg/kg/d） by gavage and intraperitoneal injection, respectively. An inhibition of tumor growth was observed. Results Tumor volume and weights decreased significantly in both groups of nude mice treated with LFWGE. In addition, the cell apoptosis rate of the LFWGE group （2 g/kg/d, 60.2%＋4.4%; 1 g/kg/d, 58.6%＋6.9%） was significantly higher than that of the control group （11.5%＋1.6%） and 5-FU group （32.1%＋3.5%） as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot method further confirmed these enhancing apoptosis and growth inhibition effects. The involvement of LFWGE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, Caspase-3, and CyclinD1. Conclusion The results showed that LFWGE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be as a natural nutrient supplements or chemopreventive agent in the treatment of human colon cancer.
文摘We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC;this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a facile way of improving the overall sensitivity with which these foreign proteins can be detected in equine plasma.
文摘Wheat blast, caused by a fungal pathogen, <i><span style="font-family:Verdana;">Magnaporthe oryzae Tritichum </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">MoT</span></i><span style="font-family:Verdana;">) pathotype (wheat isolates), was first reported in Bangladesh among Asian countries in 2016. It is a serious disease of wheat causing yield failures and significant economic losses during epidemic years. Extensive use of persistent synthetic pesticides to control notorious wheat blast imposes enormous threat to human health and environment. In addition, the increasing demand for organic food has stimulated people to look for alternative methods. Nowadays, the need for synthetic chemical-free agricultural practices is gaining importance due to effective in managing crop pests, inexpensive, biodegradable, easily available and have low toxicity to non-target organisms. In order to identify active plants, 12 kinds of methanol extracts obtained from 12 medicinal plants were conducted </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> to test the effect against </span><i><span style="font-family:Verdana;">Magnaporthe oryzae Triticum </span></i><span style="font-family:Verdana;">(</span><i><span style="font-family:Verdana;">MoT</span></i><span style="font-family:Verdana;">) pathotype. The remarkable inhibitory activity {(29.6 ± 01.5) mm, (25.1 ± 01.0) mm and (20.0 ± 02.0) mm zone of inhibition)} exhibited by the extracts (5 mg/disk) obtained from </span><i><span style="font-family:Verdana;">Artemisia indica</span></i><span style="font-family:Verdana;"> (Nagdona), </span><i><span style="font-family:Verdana;">Persicaria orientalis</span></i><span style="font-family:Verdana;"> (Bishkatali) and </span><i><span style="font-family:Verdana;">Clerodendrum indicum</span></i><span style="font-family:Verdana;"> (Bamonhati), respectively. Other medicinal plant extracts did not show any significant or no activity at all. Therefore, the three plant extracts might be a promising source for developing natural fungicides against wheat blast.</span>
