摘要
通过筛选和评价新疆植物资源的抑菌活性,为植物源农药研发提供基础。本研究通过菌丝生长速率法,以小麦根腐平脐蠕孢菌Bipolaris sorokiniana、禾谷镰刀菌Fusarium graminearum和雪腐微座孢菌Microdochium nivale为靶标菌,对13种新疆植物提取物的抑菌活性进行筛选与测定,并利用超高效液相色谱串联质谱(UPLC-MS/MS)联用技术对皱叶酸模的主要成分进行分析。结果表明,13种植物提取物对3种小麦病原菌显示出不同活性,且抑菌效果随浓度的增加而增强,其中对麦根腐平脐蠕孢菌抑制活性最强的是皱叶酸模甲醇提取物,其EC50为6.380 mg/mL;对禾谷镰刀菌抑制活性最强的是猪毛菜甲醇提取物,其EC_(50)为26.362 mg/mL;对雪腐微座孢菌抑制活性最强的是皱叶酸模乙醇提取物,其EC50值为8.203mg/mL。并采用质谱联用法分析鉴定了皱叶酸模甲醇提取物中的主要化学成分,共鉴定皱叶酸模提取物42个化学成分,主要成分为黄酮类、脂肪酰类和羧酸及其衍生物类,占总数的66.67%。因此,皱叶酸模甲醇提取物抑菌活性较好,可进一步开发应用。
Screening and evaluating the antibacterial activity of Xinjiang's plant resources provides a foundation for the development of botanical pesticides.In this study,the antifungal activities of 13 plant extracts from Xinjiang were screened and measured through the mycelial growth rate method,targeting three wheat pathogens:Bipolaris sorokiniana,Fusarium graminearum,and Microdochium nivale.Additionally,the main components of Rumex crispus were analyzed using ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The organic solvent extracts from 13 plant species showed different inhibitory activities against three wheat pathogenic fungi,and the antibacterial efficacy increased with increasing concentration.Among them,the methanol extract of Rumex crispus exhibited the strongest inhibitory activity against B.sorokiniana,with an EC50 value of 6.380 mg/mL;the methanol extract of Salsola collina demonstrated the strongest inhibitory activity against F.graminearum,with an EC50 value of 26.362 mg/mL;and the ethanol extract of R.crispus showed the strongest inhibitory activity against Microdochium nivale,with an EC50 value of 8.203 mg/mL.A total of 42 chemical components were identified in the R.crispus extract,primarily flavonoids,fatty acyls,carboxylic acids,and their derivatives,accounting for 66.67%of the total.It can be seen from this that the methanol extract of R.crispus has good antibacterial activity and is worth further study.
作者
颜彬
梁晶
龚静云
周小云
胡林峰
张军高
雷斌
李克梅
YAN Bin;LIANG Jing;GONG Jingyun;ZHOU Xiaoyun;HU Linfeng;ZHANG Jungao;LEI Bin;LI Kemei(College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Agricultural Resources and Environment,Xinjiang Academy of Agricultural Sciences/Xinjiang Crop Chemical Regulation Engineering Technology Research Center,Urumqi 830091,China)
出处
《中国生物防治学报》
北大核心
2025年第3期605-616,共12页
Chinese Journal of Biological Control
基金
新疆维吾尔自治区中央引导地方科技发展专项资金项目(ZYYD2024JD18)
中央引导地方项目“‘一带一路’国际绿色农药研制及应用技术创新基地建设”(ZYYD2023B06)
自治区科技特派员农村科技创业行动项目“春小麦高产创建关键技术集成与示范(2022BKZ022)”。
关键词
植物提取物
小麦病原菌
抑菌活性
超高效液相色谱串联质谱(UPLC-MS/MS)
plant extracts
wheat pathogenic bacteria
antibacterial activity
ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)
