摘要
生物发酵麦胚提取物在食品、饲料及医药等领域的应用受到广泛关注。目前生物转化麦胚对机体健康发挥调节作用的相关研究十分有限。该研究首先对乳酸菌发酵麦胚提取物进行了抗氧化性能和非靶向代谢组评估,进而以黑腹果蝇为模式生物,评价其对经低氧环境刺激的果蝇氧化应激状态以及肠道代谢环境的影响。结果表明,乳酸菌主要通过生物转化增加以谷胱甘肽(8.2 mg/100 mL上升至34.6 mg/100 mL)等物质为主的代谢物水平,增强体系的抗氧化潜力;作为膳食补充剂,乳酸菌发酵麦胚提取物可能通过调节缺氧果蝇肠道中参与机体能量代谢、信号转导、免疫反应等生理功能的核苷酸类、胺类、嘌呤类及生物碱类物质的丰度,改善其运动能力(85.2%上升至90.2%)和机体氧化应激状态(DPPH自由基清除能力20.3%上升至42%;ABTS阳离子自由基清除能力0.07 mmol/g上升至0.45 mmol/g),并提升了生存率(70.8%上升至86.7%)。因此,乳酸菌发酵麦胚提取物具有提高机体的缺氧耐受性的潜力。研究结果为乳酸菌发酵麦胚提取物在缓解缺氧引起的机体氧化应激中的应用提供了科学依据。
The application of biological fermented wheat embryo extract in the fields of food,feed,and medicine has garnered extensive attention.Currently,systematic studies on the regulatory effect of biotransformed wheat embryo on human health remain extremely limited.In this study,the antioxidant performance and untargeted metabolome of the fermented wheat embryo extract were comprehensively evaluated.Additionally,the impacts on the survival,exercise capacity,oxidative stress status,and intestinal metabolic environment of Drosophila melanogaster under the stimulation of a low-oxygen environment were investigated.The results demonstrated that lactic acid bacteria enhanced the antioxidant potential of the system by increasing metabolites such as glutathione(8.2 mg/100 mL to 34.6 mg/100 mL)through biotransformation.As a dietary supplement,the increased abundance of nucleotides,amines,purines,and alkaloids,which were involved in energy metabolism,signal transduction,and immune response,might contribute to the improvement of exercise capacity(85.2%to 90.2%),oxidative stress state(DPPH free radical 20.3%to 42%;ABTS cation free radical 0.07 mmol/g to 0.45 mmol/g)and the survival rate(70.8%to 86.7%).Therefore,the wheat germ extracts fermented by lactic acid bacteria have the potential to improve the hypoxia tolerance of the organism.This research provides a scientific foundation for the application of lactic acid bacteria in alleviating the oxidative stress induced by hypoxia.
作者
齐程辉
李琴丽
刘媛
孟祥龙
刘振国
郝宇
马艺荧
孔庆敏
樊哲新
韩苗
QI Chenghui;LI Qinli;LIU Yuan;MENG Xianglong;LIU Zhenguo;HAO Yu;MA Yiying;KONG Qingmin;FAN Zhexin;HAN Miao(College of Food Engineering,Harbin University of Commerce,Harbin 150000,China;Heilongjiang Green Food Science Research Institute,Harbin 150000,China;School of Food Science and Technology,Shihezi University,Shihezi 832061,China;Heilongjiang Hanfeng Environmental Detection Technology Co.Ltd.,Harbin 150000,China)
出处
《食品与发酵工业》
北大核心
2025年第19期17-25,共9页
Food and Fermentation Industries
基金
国家自然科学基金青年项目(32302051)
国家自然科学基金地区项目(32260594)
黑龙江省自然科学基金联合基金培育项目(PL2024C009)
国家级大学生创新训练计划项目(202310240091)
哈尔滨商业大学博士科研启动计划项目(22BQ83)
哈尔滨商业大学青年后备人才支持计划项目(2023-KYYWF-1056)。
关键词
乳酸菌
发酵麦胚提取物
缺氧黑腹果蝇模型
氧化应激
肠道代谢环境
lactic acid bacteria
fermented wheat germ extract
hypoxia Drosophila melanogaster model
oxidative stress
intestinal metabolic environment
