该研究以西伯利亚鲟鱼、史氏鲟鱼和海博瑞鲟鱼的肝脏铁蛋白为实验材料,成功分离纯化出3种鲟鱼肝脏铁蛋白(liver ferritin of Sturgeon,SLF),分别命名为SLF-1、SLF-2和SLF-3。通过电泳、透射电子显微镜(transmission electron microscope...该研究以西伯利亚鲟鱼、史氏鲟鱼和海博瑞鲟鱼的肝脏铁蛋白为实验材料,成功分离纯化出3种鲟鱼肝脏铁蛋白(liver ferritin of Sturgeon,SLF),分别命名为SLF-1、SLF-2和SLF-3。通过电泳、透射电子显微镜(transmission electron microscope,TEM)、傅里叶变换红外光谱(Fourier transform infrared spectroscopy,FTIR)以及氨基酸组成分析等方法,系统研究了这3种鲟鱼肝脏铁蛋白的结构和理化特性,并进行生物学信息分析,比较了它们的异同点。结果表明,SLF-1、SLF-2和SLF-3在TEM图中均表现为粒径分布均匀、外径约为12 nm的圆形颗粒;FTIR光谱分析表明,3种蛋白整体二级结构相似,但SLF-2的α螺旋的含量较低,仅为52.79%;此外,3种蛋白的总氨基酸含量均超过600 mg/g,其中,SLF-1的含量最高,SLF-3的含量最低;生物学信息分析进一步表明,SLF-1、SLF-2和SLF-3均为亲水性蛋白,属于非分泌蛋白,其亚基结构均包含4个反向平行的α螺旋,在维持蛋白结构稳定和生物体内铁平衡方面发挥重要作用。该研究为鲟鱼肝脏铁蛋白的加工与利用提供了重要的理论依据。展开更多
本研究旨在探讨鲟鱼子酱对环磷酰胺(Cyclophosphamide,CTX)诱导的免疫低下小鼠的免疫增强作用。以ICR(Institute of Cancer Research,ICR)小鼠为试验对象,腹腔注射CTX建立免疫低下模型,随后对免疫低下小鼠投喂添加低(4.2 g/kg)、中(8.4 ...本研究旨在探讨鲟鱼子酱对环磷酰胺(Cyclophosphamide,CTX)诱导的免疫低下小鼠的免疫增强作用。以ICR(Institute of Cancer Research,ICR)小鼠为试验对象,腹腔注射CTX建立免疫低下模型,随后对免疫低下小鼠投喂添加低(4.2 g/kg)、中(8.4 g/kg)、高(16.8 g/kg)剂量杂交鲟鱼子酱的鼠粮,对照组投喂不添加鱼子酱的鼠粮,为期30 d。通过H&E染色观察小鼠肠组织形态,并检测小鼠免疫器官指数、免疫细胞活力、免疫酶活和免疫因子表达水平。结果表明,与模型组相比,鱼子酱添加高剂量组能提升小鼠日采食量、体重,缓解小鼠肠绒毛损伤;高剂量鱼子酱将小鼠脾脏和胸腺器官指数、巨噬细胞吞噬能力和增殖活性显著提高了31.1%、40.1%和25.2%、48.5%(P<0.05),高剂量鱼子酱将小鼠T、B淋巴细胞增殖活性极显著提高了32.9%、50.9%(P<0.01);中剂量鱼子酱将小鼠乳酸脱氢酶、酸性磷酸酶活力显著增加了33%、13.4%(P<0.05),高剂量鱼子酱将小鼠溶菌酶含量极显著提高了53.4%(P<0.01);高剂量鱼子酱显著提升了小鼠脾脏免疫细胞因子白介素-2(Interleukin-2,IL-2)、白介素-12(Interleukin-12,IL-12)和肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)的mRNA表达水平,分别提升了86.9%、86.1%和91.5%(P<0.05),低剂量鱼子酱将白介素-6(Interleukin-6,IL-6)的mRNA表达水平显著提高了64.5%(P<0.05);此外,高剂量鱼子酱显著促进小鼠血清中IL-2、IL-12和TNF-α的分泌(P<0.05),分别上升了52.1%、30.6%和5%,中剂量鱼子酱将小鼠IL-6的分泌极显著提升了57.8%(P<0.01)。综上,鱼子酱能够减轻CTX诱导的小鼠肠道损伤,有利于肠绒毛的恢复,增强了CTX抑制的细胞免疫、体液免疫和非特异性免疫,从而增强机体的免疫调节能力。展开更多
Chondroitin sulfate(CS)is one of the main bioactive compounds in animal cartilage.In this study,the antiinflammatory activity of sturgeon-derived chondroitin sulfate(SCS)was evaluated in the dextran sulfate sodium(DSS...Chondroitin sulfate(CS)is one of the main bioactive compounds in animal cartilage.In this study,the antiinflammatory activity of sturgeon-derived chondroitin sulfate(SCS)was evaluated in the dextran sulfate sodium(DSS)-induced BALB/c mice model.Orally administration of SCS significantly alleviated the DSSinduced colitis symptoms,including the reduction of crypt depth,inhibition of the abnormal crypt foci formation,down-regulation of the proinflammatory biomarkers(NO,interleukin(IL)-6,IL-1βand tumor necrosis factor-α)and up-regulation of the anti-inflammatory biomarkers(IL-10 and IL-4).The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice,especially the increase of the relative abundance of Ruminococcaceae and Lachnospiraceae.This alternation further induced primary bile acids convert into secondary bile acids.With SCS administration,the levels of deoxycholic acid(DCA)and litho cholic acid(LCA)were increased by 1.5-and 2.5-fold,respectively.The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway,thereby suppressing the inflammatory response and attenuating inflammatory bowel disease(IBD)in mice.This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.展开更多
文摘本研究旨在探讨鲟鱼子酱对环磷酰胺(Cyclophosphamide,CTX)诱导的免疫低下小鼠的免疫增强作用。以ICR(Institute of Cancer Research,ICR)小鼠为试验对象,腹腔注射CTX建立免疫低下模型,随后对免疫低下小鼠投喂添加低(4.2 g/kg)、中(8.4 g/kg)、高(16.8 g/kg)剂量杂交鲟鱼子酱的鼠粮,对照组投喂不添加鱼子酱的鼠粮,为期30 d。通过H&E染色观察小鼠肠组织形态,并检测小鼠免疫器官指数、免疫细胞活力、免疫酶活和免疫因子表达水平。结果表明,与模型组相比,鱼子酱添加高剂量组能提升小鼠日采食量、体重,缓解小鼠肠绒毛损伤;高剂量鱼子酱将小鼠脾脏和胸腺器官指数、巨噬细胞吞噬能力和增殖活性显著提高了31.1%、40.1%和25.2%、48.5%(P<0.05),高剂量鱼子酱将小鼠T、B淋巴细胞增殖活性极显著提高了32.9%、50.9%(P<0.01);中剂量鱼子酱将小鼠乳酸脱氢酶、酸性磷酸酶活力显著增加了33%、13.4%(P<0.05),高剂量鱼子酱将小鼠溶菌酶含量极显著提高了53.4%(P<0.01);高剂量鱼子酱显著提升了小鼠脾脏免疫细胞因子白介素-2(Interleukin-2,IL-2)、白介素-12(Interleukin-12,IL-12)和肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)的mRNA表达水平,分别提升了86.9%、86.1%和91.5%(P<0.05),低剂量鱼子酱将白介素-6(Interleukin-6,IL-6)的mRNA表达水平显著提高了64.5%(P<0.05);此外,高剂量鱼子酱显著促进小鼠血清中IL-2、IL-12和TNF-α的分泌(P<0.05),分别上升了52.1%、30.6%和5%,中剂量鱼子酱将小鼠IL-6的分泌极显著提升了57.8%(P<0.01)。综上,鱼子酱能够减轻CTX诱导的小鼠肠道损伤,有利于肠绒毛的恢复,增强了CTX抑制的细胞免疫、体液免疫和非特异性免疫,从而增强机体的免疫调节能力。
文摘为研究水温对长江鲟(Acipenser dabryanus)仔稚鱼生长、免疫和消化通路基因表达的影响,本试验选取长江鲟受精卵及初孵仔稚鱼为研究对象,在16℃和19℃进行了长期孵育试验。在10 dph和100 dph时对全鱼进行转录组测序分析,并结合实时定量PCR技术筛选和验证不同时期长江鲟仔稚鱼中基因表达差异。差异表达基因(DEGs)筛选结果显示:随着生长时间推移,16℃组和19℃组的差异表达基因数量逐渐增加,G16_0 vs G19_1和G16_1 vs G19_1的DEGs数量分别为313和7719。在水温变化下,生长(MAPK信号通路、ErbB信号通路等)、免疫(趋化因子信号通路、抗原加工与递呈等)和消化(唾液分泌、蛋白质消化吸收等)系统发育相关通路等基因表达呈现显著变化,如tgf-β、fgf18、stim2、ryr1、ir、myd88stat3、socs3、pla基因等,表明这些信号通路和基因在长江鲟仔稚鱼环境温度变化下的生长、免疫和消化系统调节中有重要作用。本研究分析了水温对长江鲟的转录组调控数据,可为进一步研究鲟鱼环境适应机制提供参考和借鉴,同时为优化鲟鱼人工培育条件提供数据指导。
基金funded by grants from Beijing Fishery Innovation Team Project(BAIC07-2023-13)National Natural Science Foundation of China(32201994)。
文摘Chondroitin sulfate(CS)is one of the main bioactive compounds in animal cartilage.In this study,the antiinflammatory activity of sturgeon-derived chondroitin sulfate(SCS)was evaluated in the dextran sulfate sodium(DSS)-induced BALB/c mice model.Orally administration of SCS significantly alleviated the DSSinduced colitis symptoms,including the reduction of crypt depth,inhibition of the abnormal crypt foci formation,down-regulation of the proinflammatory biomarkers(NO,interleukin(IL)-6,IL-1βand tumor necrosis factor-α)and up-regulation of the anti-inflammatory biomarkers(IL-10 and IL-4).The gut microbiota analysis revealed that SCS alters the intestinal microbiota composition in colitis mice,especially the increase of the relative abundance of Ruminococcaceae and Lachnospiraceae.This alternation further induced primary bile acids convert into secondary bile acids.With SCS administration,the levels of deoxycholic acid(DCA)and litho cholic acid(LCA)were increased by 1.5-and 2.5-fold,respectively.The stimulated secretion of DCA and LCA showed further activation of the NF-κB signaling pathway,thereby suppressing the inflammatory response and attenuating inflammatory bowel disease(IBD)in mice.This study provided a valuable strategy for colitis prevention and treatment with sturgeon cartilage by-products.
