分析初中英语阅读教学瓶颈。阐述图式理论与ATR学习模式的内涵,并优化基于图式理论的ATR学习模式,即通过激活、积累、调试、重建、延展教学活动设计,实现知识结构化学习与迁移运用,最终实现知识、能力、素养的螺旋提升。以人教版初中《...分析初中英语阅读教学瓶颈。阐述图式理论与ATR学习模式的内涵,并优化基于图式理论的ATR学习模式,即通过激活、积累、调试、重建、延展教学活动设计,实现知识结构化学习与迁移运用,最终实现知识、能力、素养的螺旋提升。以人教版初中《英语》八年级(下)Unit 7 What is the highest mountain in the world? Section A 3a—3c为例,围绕语篇Qomolangma—the most Dangerous mountain in the world探究ATR学习模式促进学生阅读的途径。展开更多
为了探究基于衰减全反射傅里叶变换红外光谱(attenuated total reflection Fourier transform infrared spectroscopy,ATR-FTIR)技术实施农药沉积量原位感知的可能性,该研究以含有不同量广谱性杀菌剂啶酰菌胺沉积的棉花叶片为试验材料,...为了探究基于衰减全反射傅里叶变换红外光谱(attenuated total reflection Fourier transform infrared spectroscopy,ATR-FTIR)技术实施农药沉积量原位感知的可能性,该研究以含有不同量广谱性杀菌剂啶酰菌胺沉积的棉花叶片为试验材料,结合化学计量学分析方法开展相关探索。首先使用棉花叶片制成140例已知农药沉积量的标准样品,并采用ATR-FTIR技术获取其光谱数据;然后借助区间偏最小二乘法、相关性分析等方法筛选到272个相关性强的波长变量;最后以优化后的变量及偏最小二乘回归算法建立定量预测模型。结果表明模型的预测性能优异,预测的均方根误差为1.18μg/cm^(2),最低检测限(limit of detection,LOD)低至3.54μg/cm^(2);利用概率神经网络判别样品中农药沉积量是否大于LOD的整体准确率高达95%。该研究结果证明ATR-FTIR技术可实现农药沉积量的高精度原位检测,为其在生产中的应用提供理论依据和数据支撑。展开更多
Long non-coding RNAs(lncRNAs)have been implicated in cancer progression and drug resistance development.Moreover,there is evidence that lncRNA HOX transcript antisense intergenic RNA(HOTAIR)is involved in colorectal c...Long non-coding RNAs(lncRNAs)have been implicated in cancer progression and drug resistance development.Moreover,there is evidence that lncRNA HOX transcript antisense intergenic RNA(HOTAIR)is involved in colorectal cancer(CRC)progression.The present study aimed to examine the functional role of lncRNA HOTAIR in conferring radiotherapy resistance in CRC cells,as well as the underlying mechanism.The relative expression levels of HOTAIR were examined in 70 pairs of CRC tumor and para-cancerous tissues,as well as in radiosensitive and radioresistant samples.The correlations between HOTAIR expression levels and clinical features of patients with CRC were assessed using the Chi-square test.Functional assays such as cell proliferation,colony formation and apoptosis assays were conducted to determine the radiosensitivity in CRC cells with HOTAIR silencing after treatment with different doses of radiation.RNA pull-down assay andfluorescence in situ hybridization(FISH)were used to determine the interaction between HOTAIR and DNA damage response mediator ataxia-telangiectasia mutated-and Rad3-related(ATR).HOTAIR was significantly upregulated in CRC tumor tissues,especially in radioresistant tumor samples.The elevated expression of HOTAIR was correlated with more advanced histological grades,distance metastasis and the poor prognosis in patients with CRC.Silencing HOTAIR suppressed the proliferation and promoted apoptosis and radiosensitivity in CRC cells.HOTAIR knockdown also inhibited the tumorigenesis of CRC cells and enhanced the sensitivity to radiotherapy in a mouse xenograft model.Moreover,the data showed that HOTAIR could interact with ATR to regulate the DNA damage repair signaling pathway.Silencing HOTAIR impaired the ATR-ATR interacting protein(ATRIP)complex and signaling in cell cycle progression.Collectively,the present results indicate that lncRNA HOTAIR facilitates the DNA damage response pathway and promotes radioresistance in CRC cells by targeting ATR.展开更多
Background:Apolipoprotein B mRNA editing catalytic polypeptide(APOBEC),an endogenous mutator,induces DNA damage and activates the ataxia telangiectasia and Rad3-related(ATR)-checkpoint kinase 1(Chk1)pathway.Although c...Background:Apolipoprotein B mRNA editing catalytic polypeptide(APOBEC),an endogenous mutator,induces DNA damage and activates the ataxia telangiectasia and Rad3-related(ATR)-checkpoint kinase 1(Chk1)pathway.Although cisplatin-based therapy is the mainstay for muscle-invasive bladder cancer(MIBC),it has a poor survival rate.Therefore,this study aimed to evaluate the efficacy of an ATR inhibitor combined with cisplatin in the treatment of APOBEC catalytic subunit 3B(APOBEC3B)expressing MIBC.Methods:Immunohistochemical staining was performed to analyze an association between APOBEC3B and ATR in patients with MIBC.The APOBEC3B expression in MIBC cell lines was assessed using real-time polymerase chain reaction and western blot analysis.Western blot analysis was performed to confirm differences in phosphorylated Chk1(pChk1)expression according to the APOBEC3B expression.Cell viability and apoptosis analyses were performed to examine the anti-tumor activity of ATR inhibitors combined with cisplatin.Results:There was a significant association between APOBEC3B and ATR expression in the tumor tissues obtained from patients with MIBC.Cells with higher APOBEC3B expression showed higher pChk1 expression than cells expressing low APOBEC3B levels.Combination treatment of ATR inhibitor and cisplatin inhibited cell growth in MIBC cells with a higher APOBEC3B expression.Compared to cisplatin single treatment,combination treatment induced more apoptotic cell death in the cells with higher APOBEC3B expression.Conclusion:Our study shows that APOBEC3B’s higher expression status can enhance the sensitivity of MIBC to cisplatin upon ATR inhibition.This result provides new insight into appropriate patient selection for the effective application of ATR inhibitors in MIBC.展开更多
文摘分析初中英语阅读教学瓶颈。阐述图式理论与ATR学习模式的内涵,并优化基于图式理论的ATR学习模式,即通过激活、积累、调试、重建、延展教学活动设计,实现知识结构化学习与迁移运用,最终实现知识、能力、素养的螺旋提升。以人教版初中《英语》八年级(下)Unit 7 What is the highest mountain in the world? Section A 3a—3c为例,围绕语篇Qomolangma—the most Dangerous mountain in the world探究ATR学习模式促进学生阅读的途径。
文摘为了探究基于衰减全反射傅里叶变换红外光谱(attenuated total reflection Fourier transform infrared spectroscopy,ATR-FTIR)技术实施农药沉积量原位感知的可能性,该研究以含有不同量广谱性杀菌剂啶酰菌胺沉积的棉花叶片为试验材料,结合化学计量学分析方法开展相关探索。首先使用棉花叶片制成140例已知农药沉积量的标准样品,并采用ATR-FTIR技术获取其光谱数据;然后借助区间偏最小二乘法、相关性分析等方法筛选到272个相关性强的波长变量;最后以优化后的变量及偏最小二乘回归算法建立定量预测模型。结果表明模型的预测性能优异,预测的均方根误差为1.18μg/cm^(2),最低检测限(limit of detection,LOD)低至3.54μg/cm^(2);利用概率神经网络判别样品中农药沉积量是否大于LOD的整体准确率高达95%。该研究结果证明ATR-FTIR技术可实现农药沉积量的高精度原位检测,为其在生产中的应用提供理论依据和数据支撑。
基金This study was supported by the Inner Mongolia Science and Technology Department Science and Technology Research Project(No.2021GG0270)National Natural Science Foundation of China(81860534)+5 种基金Natural Science Foundation of Inner Mongolia(2021MS08152)Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(NJYT22004)Scientific and Technological Innovative Research Team for Inner Mongolia Medical University of Transformation Application of Organoid in Medical and Industrial Interdiscipline(YKD2022TD002)Major Project of Inner Mongolia Medical University(YKD2022 ZD002)Radiobiology System and Team Construction of Radiotherapy for Inner Mongolia Medical University(YKD2022XK014)Key Laboratoy of Radiation Physics and Biology of Inner Mongolia Medical University(PIKY2023030).
文摘Long non-coding RNAs(lncRNAs)have been implicated in cancer progression and drug resistance development.Moreover,there is evidence that lncRNA HOX transcript antisense intergenic RNA(HOTAIR)is involved in colorectal cancer(CRC)progression.The present study aimed to examine the functional role of lncRNA HOTAIR in conferring radiotherapy resistance in CRC cells,as well as the underlying mechanism.The relative expression levels of HOTAIR were examined in 70 pairs of CRC tumor and para-cancerous tissues,as well as in radiosensitive and radioresistant samples.The correlations between HOTAIR expression levels and clinical features of patients with CRC were assessed using the Chi-square test.Functional assays such as cell proliferation,colony formation and apoptosis assays were conducted to determine the radiosensitivity in CRC cells with HOTAIR silencing after treatment with different doses of radiation.RNA pull-down assay andfluorescence in situ hybridization(FISH)were used to determine the interaction between HOTAIR and DNA damage response mediator ataxia-telangiectasia mutated-and Rad3-related(ATR).HOTAIR was significantly upregulated in CRC tumor tissues,especially in radioresistant tumor samples.The elevated expression of HOTAIR was correlated with more advanced histological grades,distance metastasis and the poor prognosis in patients with CRC.Silencing HOTAIR suppressed the proliferation and promoted apoptosis and radiosensitivity in CRC cells.HOTAIR knockdown also inhibited the tumorigenesis of CRC cells and enhanced the sensitivity to radiotherapy in a mouse xenograft model.Moreover,the data showed that HOTAIR could interact with ATR to regulate the DNA damage repair signaling pathway.Silencing HOTAIR impaired the ATR-ATR interacting protein(ATRIP)complex and signaling in cell cycle progression.Collectively,the present results indicate that lncRNA HOTAIR facilitates the DNA damage response pathway and promotes radioresistance in CRC cells by targeting ATR.
基金supported by St.Vincent’s Hospital,the Research Institute of Medical Science(Grant Number:SVHR-2021-03).
文摘Background:Apolipoprotein B mRNA editing catalytic polypeptide(APOBEC),an endogenous mutator,induces DNA damage and activates the ataxia telangiectasia and Rad3-related(ATR)-checkpoint kinase 1(Chk1)pathway.Although cisplatin-based therapy is the mainstay for muscle-invasive bladder cancer(MIBC),it has a poor survival rate.Therefore,this study aimed to evaluate the efficacy of an ATR inhibitor combined with cisplatin in the treatment of APOBEC catalytic subunit 3B(APOBEC3B)expressing MIBC.Methods:Immunohistochemical staining was performed to analyze an association between APOBEC3B and ATR in patients with MIBC.The APOBEC3B expression in MIBC cell lines was assessed using real-time polymerase chain reaction and western blot analysis.Western blot analysis was performed to confirm differences in phosphorylated Chk1(pChk1)expression according to the APOBEC3B expression.Cell viability and apoptosis analyses were performed to examine the anti-tumor activity of ATR inhibitors combined with cisplatin.Results:There was a significant association between APOBEC3B and ATR expression in the tumor tissues obtained from patients with MIBC.Cells with higher APOBEC3B expression showed higher pChk1 expression than cells expressing low APOBEC3B levels.Combination treatment of ATR inhibitor and cisplatin inhibited cell growth in MIBC cells with a higher APOBEC3B expression.Compared to cisplatin single treatment,combination treatment induced more apoptotic cell death in the cells with higher APOBEC3B expression.Conclusion:Our study shows that APOBEC3B’s higher expression status can enhance the sensitivity of MIBC to cisplatin upon ATR inhibition.This result provides new insight into appropriate patient selection for the effective application of ATR inhibitors in MIBC.
