The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point...The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point-of-care testing(POCT)has emerged as a promising alternative,which is awaiting rigorous validation.We assessed 226 samples from patients at the First Affiliated Hospital of Guangzhou Medical University using a Beckman Coulter AU5821 and a PUSHKANG POCT Biochemistry Analyzer MS100.Furthermore,350 samples were evaluated with a Stago coagulation analyzer STAR MAX and a PUSHKANG POCT Coagulation Analyzer MC100.Metrics included thirteen biochemical indexes,such as albumin,and five coagulation indices,such as prothrombin time.Comparisons were drawn against the PUSHKANG POCT analyzer.Bland-Altman plots(MS100:0.8206-0.9995;MC100:0.8318-0.9911)evinced significant consistency between methodologies.Spearman correlation pinpointed a potent linear association between conventional devices and the PUSHKANG POCT analyzer,further underscored by a robust correlation coefficient(MS100:0.713-0.949;MC100:0.593-0.950).The PUSHKANG POCT was validated as a dependable tool for serum and whole blood biochemical and coagulation diagnostics.This emphasizes its prospective clinical efficacy,offering clinicians a swift diagnostic tool and heralding a new era of enhanced patient care outcomes.展开更多
细菌CRISPR-Cas系统的综合应用胡祐等[1]重点介绍了细菌的CRISPR-Cas系统凭借高特异性锚定靶核酸和高活性反式切割能力,有望成为特异性好、灵敏度高的信号报告系统。可视化检测是通过肉眼即可判断结果,无需依赖高能光源及复杂的光学检...细菌CRISPR-Cas系统的综合应用胡祐等[1]重点介绍了细菌的CRISPR-Cas系统凭借高特异性锚定靶核酸和高活性反式切割能力,有望成为特异性好、灵敏度高的信号报告系统。可视化检测是通过肉眼即可判断结果,无需依赖高能光源及复杂的光学检测系统,可极大程度上简化核酸发展即时检测(point of care testing,POCT)系统。CRISPR-Cas系统酶促底物显色法检测中常用的酶包括以辣根过氧化物酶、脱氧核酶等为代表的过氧化物酶和半乳糖苷酶。展开更多
基金supported by the National Natural Science Foundation of China(No.82302607)the Guangdong Basic and Applied Basic Research Foundation(Nos.2022A1515110555 and 2023A1515010932)+3 种基金the State Key Laboratory of Respiratory Disease(No.SKLRD-Z-202410)the Precision Medicine Joint Foundation of Basic and Applied Basic Research Foundation of Guangdong Province(No.2021B1515230008)the Independent Project of Guangdong-Hong Kong-Macao Joint Laboratory for Respiratory Infectious Diseases(No.GHMJLRID-Z-202102)the Guangdong Zhong Nanshan Medical Foundation(No.ZNSXS-20220015).
文摘The accurate and timely detection of biochemical coagulation indicators is pivotal in pulmonary and critical care medicine.Despite their reliability,traditional laboratories often lag in terms of rapid diagnosis.Point-of-care testing(POCT)has emerged as a promising alternative,which is awaiting rigorous validation.We assessed 226 samples from patients at the First Affiliated Hospital of Guangzhou Medical University using a Beckman Coulter AU5821 and a PUSHKANG POCT Biochemistry Analyzer MS100.Furthermore,350 samples were evaluated with a Stago coagulation analyzer STAR MAX and a PUSHKANG POCT Coagulation Analyzer MC100.Metrics included thirteen biochemical indexes,such as albumin,and five coagulation indices,such as prothrombin time.Comparisons were drawn against the PUSHKANG POCT analyzer.Bland-Altman plots(MS100:0.8206-0.9995;MC100:0.8318-0.9911)evinced significant consistency between methodologies.Spearman correlation pinpointed a potent linear association between conventional devices and the PUSHKANG POCT analyzer,further underscored by a robust correlation coefficient(MS100:0.713-0.949;MC100:0.593-0.950).The PUSHKANG POCT was validated as a dependable tool for serum and whole blood biochemical and coagulation diagnostics.This emphasizes its prospective clinical efficacy,offering clinicians a swift diagnostic tool and heralding a new era of enhanced patient care outcomes.
文摘细菌CRISPR-Cas系统的综合应用胡祐等[1]重点介绍了细菌的CRISPR-Cas系统凭借高特异性锚定靶核酸和高活性反式切割能力,有望成为特异性好、灵敏度高的信号报告系统。可视化检测是通过肉眼即可判断结果,无需依赖高能光源及复杂的光学检测系统,可极大程度上简化核酸发展即时检测(point of care testing,POCT)系统。CRISPR-Cas系统酶促底物显色法检测中常用的酶包括以辣根过氧化物酶、脱氧核酶等为代表的过氧化物酶和半乳糖苷酶。
