摘要
在当前对快速、安全、便捷的核酸检测技术需求日益增长的背景下,借鉴乙醇纯化核酸的过程及微滤器的过滤原理,设计出利用核酸沉淀阻塞通道的目视检测微流控芯片。在目标核酸特异性扩增后,使用乙醇进行沉淀,基于多孔介质内非刚性颗粒对流动的物理阻塞机理,实现目标核酸的目视检测。使用PMMA微球作为微流控芯片中的阻塞结构,30组重复实验全部在10 min内成功检出,保证了该方案的可行性。该方法兼容恒温和非恒温核酸扩增技术,芯片内环境全程负压且多次进行酒精处理,提供双重安全保障,提高了POCT应用的安全性。
With the increasing demand for rapid,safe,and convenient nucleic acid detection technologies,this study designs a visual detection microfluidic chip based on the principle of nucleic acid precipitation and channel blockage.Inspired by the ethanol purification process of nucleic acids and the filtration principle of microfilters,this method utilizes the physical blockage effect of precipitated nucleic acids to achieve visual detection.After specific amplification of the target nucleic acids,ethanol is used to precipitate them,thereby enabling visual detection of the target nucleic acid.We designed the microfluidic chip using poly(methyl methacrylate)(PMMA)microspheres as the porous medium.Thirty repeated experiments were conducted on this microfluidic chip,all of which successfully detected the target within 10 minutes,demonstrating practicality and high accuracy.This qualitative reading method is applicable to all pathogens in principle and is compatible with both isothermal and non-isothermal amplification techniques.Additionally,the chip’s internal environment is maintained under negative pressure throughout the process,and it is treated multiple times with alcohol,providing dual safety measures and enhancing the safety of its application in point-of-care testing(POCT).
作者
张文普
陈汾琳
李雯曦
马晟利
葛文玉
李彪
ZHANG Wenpu;CHEN Fenlin;LI Wenxi;MA Shengli;GE Wenyu;LI Biao(School of Energy Science and Engineering,Harbin Institute of Technology,Harbin 150001,Heilongjiang,China;School of Medicine and Health,Harbin Institute of Technology,Harbin 150001,Heilongjiang,China;Heilongjiang Provincial Hospital,Harbin 150001,Heilongjiang,China)
出处
《化工学报》
北大核心
2025年第11期5799-5805,共7页
CIESC Journal
基金
黑龙江省新型冠状病毒感染的肺炎疫情应急防治诊疗技术研究项目(GA20C003)。
关键词
微流控
核酸检测
POCT
核酸纯化
病毒
microfluids
nucleic acid testing(NAT)
point-of-care testing(POCT)
nucleic acid purification
virus
