[Objective] This study aimed to explore the synergistic effect of oligochi- tosan and EM bacteria and provide a theoretical basis for its application in animal health, aquaculture, sewage treatment and other fields. [...[Objective] This study aimed to explore the synergistic effect of oligochi- tosan and EM bacteria and provide a theoretical basis for its application in animal health, aquaculture, sewage treatment and other fields. [Method] Different concentrations of oligochitosan were added into EM bacteria liquid and the stability was preliminarily studied. [Result] Addition of oligochitosan with appropriate proportion had played a supporting role in the stable storage of EM bacteria liquid and had shown certain inhibition effect on flatulence of EM bacteria liquid. Addition of oligochitosan had no significant effect on the quality and re-fermentation of EM bacteria. [Conclusion] The study indicated that oligochitosan could be utilized in conjunction with EM bacteria liquid, having a promising practical potential application.展开更多
Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated...Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.展开更多
This study investigated the antiviral activity and molecular mechanisms of oligochitosan against potato virus Y(PVY)in Nicotiana benthamiana.The results demonstrate that oligochitosan exhibits significant anti-PVY act...This study investigated the antiviral activity and molecular mechanisms of oligochitosan against potato virus Y(PVY)in Nicotiana benthamiana.The results demonstrate that oligochitosan exhibits significant anti-PVY activity,achieving a preventive efficacy of 54.7%.Biochemical analyses revealed that oligochitosan treatment enhances the activities of defense-related enzymes and stimulates hydrogen peroxide accumulation in N.benthamiana.Integrated transcriptomic and proteomic analyses identified key differentially expressed genes associated with reactive oxygen species signaling and the mitogenactivated protein kinase pathway,including PYL1,PP2C,OXI1,NDPK4,MAPKKK21 and POD4.Functional characterization demonstrated that oligochitosan specifically upregulates OXI1 expression while enhancing MAPKKK21 and NDPK4 transcript levels,thereby conferring enhanced PVY resistance.These findings establish that oligochitosan-induced plant defense against PVY operates primarily through ROS-mediated activation of the mitogen-activated protein kinase signaling cascade.This work provides novel insights into the molecular basis of the antiviral activity of oligochitosan in plant protection.展开更多
文摘[Objective] This study aimed to explore the synergistic effect of oligochi- tosan and EM bacteria and provide a theoretical basis for its application in animal health, aquaculture, sewage treatment and other fields. [Method] Different concentrations of oligochitosan were added into EM bacteria liquid and the stability was preliminarily studied. [Result] Addition of oligochitosan with appropriate proportion had played a supporting role in the stable storage of EM bacteria liquid and had shown certain inhibition effect on flatulence of EM bacteria liquid. Addition of oligochitosan had no significant effect on the quality and re-fermentation of EM bacteria. [Conclusion] The study indicated that oligochitosan could be utilized in conjunction with EM bacteria liquid, having a promising practical potential application.
文摘Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO2) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO2 was prepared by dispersion of nSiO2 in OC solution. The morphology of nSiO2 in the mixture of OC-nSiO2 was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO2 on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO2 with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO2 hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO2 hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.
基金financial support from the National Key Research and Development Program of China(2023YFD1700505).
文摘This study investigated the antiviral activity and molecular mechanisms of oligochitosan against potato virus Y(PVY)in Nicotiana benthamiana.The results demonstrate that oligochitosan exhibits significant anti-PVY activity,achieving a preventive efficacy of 54.7%.Biochemical analyses revealed that oligochitosan treatment enhances the activities of defense-related enzymes and stimulates hydrogen peroxide accumulation in N.benthamiana.Integrated transcriptomic and proteomic analyses identified key differentially expressed genes associated with reactive oxygen species signaling and the mitogenactivated protein kinase pathway,including PYL1,PP2C,OXI1,NDPK4,MAPKKK21 and POD4.Functional characterization demonstrated that oligochitosan specifically upregulates OXI1 expression while enhancing MAPKKK21 and NDPK4 transcript levels,thereby conferring enhanced PVY resistance.These findings establish that oligochitosan-induced plant defense against PVY operates primarily through ROS-mediated activation of the mitogen-activated protein kinase signaling cascade.This work provides novel insights into the molecular basis of the antiviral activity of oligochitosan in plant protection.
