Background:Melanoma is a deadly skin tumor resulting from the malignant transformation of melanocytes.It is highly malignant and invasive,with the highest mortality rate among skin cancers.Acalypha australis L.(AAL),a...Background:Melanoma is a deadly skin tumor resulting from the malignant transformation of melanocytes.It is highly malignant and invasive,with the highest mortality rate among skin cancers.Acalypha australis L.(AAL),a plant with dual medicinal and culinary purposes,is commonly regarded as an edible wild vegetable in southern China.Additionally,AAL has a long history of medicinal use in China,often employed for its hemostatic,anti-diarrheal,and anti-inflammatory properties.Modern pharmacology has demonstrated that AAL possesses functions such as weight loss,antimicrobial activity,antiviral effects,and treatment for ulcerative colitis.However,there is currently no research available regarding its effectiveness and mechanisms of action on melanoma.Methods:In this investigation,we used methyl thiazolyl tetrazolium assay to detect cell viability,transwell assay to detect cell migration and invasion ability,and Western blot assay to detect relevant signaling pathways.Results:The present study reveals that 2 mg/mL AAL effectively suppresses the metastasis of B16 cells,while simultaneously triggering the expression of key apoptosis-related proteins,including Bcl-2,Bax,and cleaved caspased 3.Subsequent investigations demonstrate that AAL exerts this inhibitory effect via the PI3K/AKT signal transduction pathway,as evidenced by the observed deficits in Ras,AKT,p-AKT,and PI3K expression levels.Conclusion:These findings indicated that AAL could be a valuable therapeutic option for reducing the metastatic potential of B16 melanoma cells.展开更多
Objective:To investigate the effects of an aqueous leaf extract of Acalypha wilkesiana (A.wilkesiana) on plasma chemistry and hematological indices of sub-chronic salt-loaded rate. Method:The control group received a ...Objective:To investigate the effects of an aqueous leaf extract of Acalypha wilkesiana (A.wilkesiana) on plasma chemistry and hematological indices of sub-chronic salt-loaded rate. Method:The control group received a diet consisting 100%of the commercial feed,while the four test groups were received a diet consisting 8%salt and 92%commercial feed all through, except for the reference treatment group that had its salt-loading discontinued after six weeks. The extract was orally administered daily at 200 and 250 mg/kg body weight;while the test control,reference and control groups received appropriate volumes of water by the same route. Results:The extract had no negative effects on markers of liver and kidney functions,produced hemoconcentration,significantly higher(P【0.05) plasma calcium and potassium levels,and significantly lower(P【0.05) plasma sodium and chloride levels in the test animals compared to test control.Conclusions:This result supports the traditional use of A.wilkesiana in the management of hypertension and suggesls that the extract may be a potassium sparing diuretic whose mechanism of antihypertensive action may be via alteration of plasma sodium and potassium balances or calcium mediated alteration in vascular muscle tone.展开更多
Objective:To investigate the protective effect of aqueous leaf extract of Acalypha(A.)wilkesiana Muell.Arg(Euphorbiaceae)against cyclophosphamide-induced toxicity in albino rats.Methods:Twenty male albino rats were ra...Objective:To investigate the protective effect of aqueous leaf extract of Acalypha(A.)wilkesiana Muell.Arg(Euphorbiaceae)against cyclophosphamide-induced toxicity in albino rats.Methods:Twenty male albino rats were randomly divided into five groups of four animals each.The control group(Ⅰ)was fed with pellets and distilled water,while groupⅡwas orally administered with only 20 mg/kg cyclophosphamide.GroupsⅢ,ⅣandⅤwere coadministered with 20 mg/kg body weight cyclophosphamide and 110,220 and 440 mg/kg body weight A.wilkesiana leaf extract,respectively,for 7 d.After treatment,liver and kidney function biomarkers,haematological parameters,liver antioxidants,and mitochondrial membrane permeability transition pore opening were investigated.Results:A.wilkesiana leaf extract significantly reduced(P<0.05)cyclophosphamide-induced increase in plasma aspartate aminotransferase,alanine aminotransferase,creatinine,uric acid and urea.It increased superoxide dismutase,catalase,glutathione-S-transferase activities and reduced glutathione levels.It also increased packed cell volume count,hemoglobin concentration and white blood cell count while inhibiting the induction of mitochondrial swelling.Conclusions:This study demonstrates that aqueous extract of A.wilkesiana leaf protected tissues against cyclophosphamide-induced oxidative damage.展开更多
The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator p...The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator parameters in Rats. The study was carried out in the College of Health Sciences, Department of Hematology, Igbinedion University, Okada between the Month of June and July, 2012. Following 30 days post-oral administration of extracts on (Group 2 and Group 3), exercise training on (Group 2 and Group 4) and No treated on Group 1, hematological parameters were determined using the sysmex®?Automated Hematological Analyzer, while CD4 and CD8 cells were estimated using Partec cyflow counter and serum IgG is determined using the ELISA Method. CD4 cells, CD8, and Total WBC count show a statistically significant increase while Hb concentration and IgG level show a statistically significant decrease in Group 2 and Group 4 (P < 0.05). Total WBC count shows a statistically significant increase against the control Group, while CD4 cell, CD8 cell count, Hb-concentration and IgG level show a statistically insignificant increase (P > 0.05), Table 2. Acalypha wilkesiana at a concentration of 300 mg/g/day seems to be immuno-protective in Rats. Single or combine effects of prolonged exercise and A. wilkesiana produce significant change in some immuno indicators parameters. However, the molecular mechanism behind their combined effect would require further investigation.展开更多
基金This work was supported by the Hunan Education Department Project(NO.20A390)National Innovation and Entrepreneurship Training Program(S202010548007).
文摘Background:Melanoma is a deadly skin tumor resulting from the malignant transformation of melanocytes.It is highly malignant and invasive,with the highest mortality rate among skin cancers.Acalypha australis L.(AAL),a plant with dual medicinal and culinary purposes,is commonly regarded as an edible wild vegetable in southern China.Additionally,AAL has a long history of medicinal use in China,often employed for its hemostatic,anti-diarrheal,and anti-inflammatory properties.Modern pharmacology has demonstrated that AAL possesses functions such as weight loss,antimicrobial activity,antiviral effects,and treatment for ulcerative colitis.However,there is currently no research available regarding its effectiveness and mechanisms of action on melanoma.Methods:In this investigation,we used methyl thiazolyl tetrazolium assay to detect cell viability,transwell assay to detect cell migration and invasion ability,and Western blot assay to detect relevant signaling pathways.Results:The present study reveals that 2 mg/mL AAL effectively suppresses the metastasis of B16 cells,while simultaneously triggering the expression of key apoptosis-related proteins,including Bcl-2,Bax,and cleaved caspased 3.Subsequent investigations demonstrate that AAL exerts this inhibitory effect via the PI3K/AKT signal transduction pathway,as evidenced by the observed deficits in Ras,AKT,p-AKT,and PI3K expression levels.Conclusion:These findings indicated that AAL could be a valuable therapeutic option for reducing the metastatic potential of B16 melanoma cells.
文摘Objective:To investigate the effects of an aqueous leaf extract of Acalypha wilkesiana (A.wilkesiana) on plasma chemistry and hematological indices of sub-chronic salt-loaded rate. Method:The control group received a diet consisting 100%of the commercial feed,while the four test groups were received a diet consisting 8%salt and 92%commercial feed all through, except for the reference treatment group that had its salt-loading discontinued after six weeks. The extract was orally administered daily at 200 and 250 mg/kg body weight;while the test control,reference and control groups received appropriate volumes of water by the same route. Results:The extract had no negative effects on markers of liver and kidney functions,produced hemoconcentration,significantly higher(P【0.05) plasma calcium and potassium levels,and significantly lower(P【0.05) plasma sodium and chloride levels in the test animals compared to test control.Conclusions:This result supports the traditional use of A.wilkesiana in the management of hypertension and suggesls that the extract may be a potassium sparing diuretic whose mechanism of antihypertensive action may be via alteration of plasma sodium and potassium balances or calcium mediated alteration in vascular muscle tone.
文摘Objective:To investigate the protective effect of aqueous leaf extract of Acalypha(A.)wilkesiana Muell.Arg(Euphorbiaceae)against cyclophosphamide-induced toxicity in albino rats.Methods:Twenty male albino rats were randomly divided into five groups of four animals each.The control group(Ⅰ)was fed with pellets and distilled water,while groupⅡwas orally administered with only 20 mg/kg cyclophosphamide.GroupsⅢ,ⅣandⅤwere coadministered with 20 mg/kg body weight cyclophosphamide and 110,220 and 440 mg/kg body weight A.wilkesiana leaf extract,respectively,for 7 d.After treatment,liver and kidney function biomarkers,haematological parameters,liver antioxidants,and mitochondrial membrane permeability transition pore opening were investigated.Results:A.wilkesiana leaf extract significantly reduced(P<0.05)cyclophosphamide-induced increase in plasma aspartate aminotransferase,alanine aminotransferase,creatinine,uric acid and urea.It increased superoxide dismutase,catalase,glutathione-S-transferase activities and reduced glutathione levels.It also increased packed cell volume count,hemoglobin concentration and white blood cell count while inhibiting the induction of mitochondrial swelling.Conclusions:This study demonstrates that aqueous extract of A.wilkesiana leaf protected tissues against cyclophosphamide-induced oxidative damage.
文摘The aim of this study is to compare the effects of single and combined oral administration of fresh aqueous extracts of Acalypha wilkesiana supplementation at 300 mg/ml and exercise training on some immuno indicator parameters in Rats. The study was carried out in the College of Health Sciences, Department of Hematology, Igbinedion University, Okada between the Month of June and July, 2012. Following 30 days post-oral administration of extracts on (Group 2 and Group 3), exercise training on (Group 2 and Group 4) and No treated on Group 1, hematological parameters were determined using the sysmex®?Automated Hematological Analyzer, while CD4 and CD8 cells were estimated using Partec cyflow counter and serum IgG is determined using the ELISA Method. CD4 cells, CD8, and Total WBC count show a statistically significant increase while Hb concentration and IgG level show a statistically significant decrease in Group 2 and Group 4 (P < 0.05). Total WBC count shows a statistically significant increase against the control Group, while CD4 cell, CD8 cell count, Hb-concentration and IgG level show a statistically insignificant increase (P > 0.05), Table 2. Acalypha wilkesiana at a concentration of 300 mg/g/day seems to be immuno-protective in Rats. Single or combine effects of prolonged exercise and A. wilkesiana produce significant change in some immuno indicators parameters. However, the molecular mechanism behind their combined effect would require further investigation.
