摘要
目的:考察铁苋菜的抗氧化及抗NO作用,对铁苋菜治疗UC模型机制进行初步探讨。方法:生化方法测定模型对照组、SPSA阳性对照药组、铁苋菜给药高、中、低剂量组大鼠肠组织中SOD、MDA、GSH-Px以及NO、iNOS的水平;RT-PCR方法测定正常组、模型组及铁苋菜中剂量给药组大鼠肠组织中iNOS mRNA的表达。结果:铁苋菜中高剂量给药可以显著降低模型组肠组织中MDA的水平,显著升高SOD、GSH-Px的水平;铁苋菜中、高剂量可以显著降低模型大鼠NO及iNOS水平。并且铁苋菜中剂量给药组的iNOS mRNA的表达显著低于模型组。结论:铁苋菜具有抗氧化和抗NO作用,铁苋菜通过抑制iNOS基因表达抑制iNOS,减少NO过量生成,是铁苋菜发挥抗UC作用的机制之一。
AIM： To investigate the antioxidative and antinitrosative effects of copperleaf （ Acalypha australis L） on TNBS-induced ulcerative colitis rats. METHODS： Using biochemical method, the activity of antioxidative enzyme SOD, GSH-Px and MDA were determined in normal, model and three rat＇ s groups with low, moderate and high copperleaf decoction respectively. At the same time, the production of NO and the activity of iNOS were also measured. The expression of iNOS mRNA was also determined in normal, model and moderate-copperleaf decoction rats groups through RT-PCR method. RESULTS： The index SOD and GSH-Px increased and MDA reduced signif- icantly in high-and moderate-copperleaf decoction groups compared with the model group. The production of NO the activity of iNOS reduced significantly in high-and moderate-copperleaf decoction groups compared with the model group. RT-PCR results demonstrated that the expression of iNOS mRNA was significantly inhibited in TNBS-induced ulcerative colitis rats after being treated with moderate-dosage copperleaf. CONCLUSION： Acalypha austrails L. has antioxidative and antinitrosative effects which is probably one of the mechanism of copperleaf for treating UC.
出处
《中成药》
CAS
CSCD
北大核心
2007年第1期36-40,共5页
Chinese Traditional Patent Medicine
