GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI ...GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene展开更多
Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ...Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.展开更多
This study demonstrates a simple 2-bit phased array operating at 27 GHz that supports one-dimensional beam scanning with left-handed circular polarization(LHCP).The antenna is constructed using a compact four-layer pr...This study demonstrates a simple 2-bit phased array operating at 27 GHz that supports one-dimensional beam scanning with left-handed circular polarization(LHCP).The antenna is constructed using a compact four-layer printed circuit board(PCB)structure,consisting of a 90°phase shifter layer with microstrip structures,a ground(GND)layer,a direct current(DC)control layer,and a circularly polarized annular radiation patch layer with 1-bit phase shifting.Based on the proposed unit structure,a 1×8 array with half-wavelength inter-element spacing was designed and validated.Experimental results show that the array achieves a peak gain of 10.23 dBi and is capable of beam scanning within±50°.展开更多
HTTP Adaptive Streaming(HAS)of video content is becoming an undivided part of the Internet and accounts for most of today’s network traffic.Video compression technology plays a vital role in efficiently utilizing net...HTTP Adaptive Streaming(HAS)of video content is becoming an undivided part of the Internet and accounts for most of today’s network traffic.Video compression technology plays a vital role in efficiently utilizing network channels,but encoding videos into multiple representations with selected encoding parameters is a significant challenge.However,video encoding is a computationally intensive and time-consuming operation that requires high-performance resources provided by on-premise infrastructures or public clouds.In turn,the public clouds,such as Amazon elastic compute cloud(EC2),provide hundreds of computing instances optimized for different purposes and clients’budgets.Thus,there is a need for algorithms and methods for optimized computing instance selection for specific tasks such as video encoding and transcoding operations.Additionally,the encoding speed directly depends on the selected encoding parameters and the complexity characteristics of video content.In this paper,we first benchmarked the video encoding performance of Amazon EC2 spot instances using multiple×264 codec encoding parameters and video sequences of varying complexity.Then,we proposed a novel fast approach to optimize Amazon EC2 spot instances and minimize video encoding costs.Furthermore,we evaluated how the optimized selection of EC2 spot instances can affect the encoding cost.The results show that our approach,on average,can reduce the encoding costs by at least 15.8%and up to 47.8%when compared to a random selection of EC2 spot instances.展开更多
目的建立针对高致病性2型猪链球菌（Streptococcus suis 2，SS2）89K毒力岛Ⅳ型分泌系统的环介导等温扩增（100p-mediated isothermal amplification，LAMP）方法。方法根据国内流行株特有89K毒力岛编码的Ⅳ型分泌系统（T4SS-89K）的vir...目的建立针对高致病性2型猪链球菌（Streptococcus suis 2，SS2）89K毒力岛Ⅳ型分泌系统的环介导等温扩增（100p-mediated isothermal amplification，LAMP）方法。方法根据国内流行株特有89K毒力岛编码的Ⅳ型分泌系统（T4SS-89K）的virB4-89K基因保守区域设计并合成LAMP引物，通过优化反应体系和扩增条件建立T4SS-89K快速检测方法，对方法的特异性、敏感性进行评估。结果优化的LAMP反应体系具有良好的扩增效率，检测灵敏度为1．53×10^-1拷贝／反应，全部扩增检测可在60rain内完成；建立的LAMP法具有良好的特异性，与不含T4SS-89K的猪链球菌（包括1／2型、1型、3-33型），以及其他常见9种对照菌均不发生特异性扩增，而与1998和2005年疫情现场分离的高致病性SS2均可发生特异性扩增。结论建立的LAMP方法具有特异、灵敏，设备要求简单等特点，可用于高致病性SS2快速检测。展开更多
[目的]检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV ...[目的]检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV encoded small RNA-1,EBER-1)。免疫组织化学的方法检测EBER-1阳性和阴性肺癌组织中LMP-1、p53、Bcl-2及MMP-9的表达。[结果]108例肺癌组织中EBER-1阳性36例,阳性率33.3%;22例癌旁正常肺组织中EBER-1阳性1例,阳性率4.5%,差异有统计学意义(P<0.01)。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1%和4.2%,差异无统计学意义(P>0.05);在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积(AA)和积分光密度(IOD)均高于阴性组,差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9AA和IOD均高于阴性组,但差异无统计学意义(P>0.05)。[结论]EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达,进而在肺癌的发生、发展和转移中发挥作用。展开更多
文摘GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene
基金funded by the National Key Research and Development Program of China(Nos.2019YFE0190900,2018YFD0900705).
文摘Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.
基金supported in part by the National Natural Science Foundation of China under Grant No.62171103in part by the National Natural Science Foundation of China“111”Project under Grant No.BP0719011.
文摘This study demonstrates a simple 2-bit phased array operating at 27 GHz that supports one-dimensional beam scanning with left-handed circular polarization(LHCP).The antenna is constructed using a compact four-layer printed circuit board(PCB)structure,consisting of a 90°phase shifter layer with microstrip structures,a ground(GND)layer,a direct current(DC)control layer,and a circularly polarized annular radiation patch layer with 1-bit phase shifting.Based on the proposed unit structure,a 1×8 array with half-wavelength inter-element spacing was designed and validated.Experimental results show that the array achieves a peak gain of 10.23 dBi and is capable of beam scanning within±50°.
基金This work has been supported in part by the Austrian Research Promotion Agency(FFG)under the APOLLO and Karnten Fog project.
文摘HTTP Adaptive Streaming(HAS)of video content is becoming an undivided part of the Internet and accounts for most of today’s network traffic.Video compression technology plays a vital role in efficiently utilizing network channels,but encoding videos into multiple representations with selected encoding parameters is a significant challenge.However,video encoding is a computationally intensive and time-consuming operation that requires high-performance resources provided by on-premise infrastructures or public clouds.In turn,the public clouds,such as Amazon elastic compute cloud(EC2),provide hundreds of computing instances optimized for different purposes and clients’budgets.Thus,there is a need for algorithms and methods for optimized computing instance selection for specific tasks such as video encoding and transcoding operations.Additionally,the encoding speed directly depends on the selected encoding parameters and the complexity characteristics of video content.In this paper,we first benchmarked the video encoding performance of Amazon EC2 spot instances using multiple×264 codec encoding parameters and video sequences of varying complexity.Then,we proposed a novel fast approach to optimize Amazon EC2 spot instances and minimize video encoding costs.Furthermore,we evaluated how the optimized selection of EC2 spot instances can affect the encoding cost.The results show that our approach,on average,can reduce the encoding costs by at least 15.8%and up to 47.8%when compared to a random selection of EC2 spot instances.
文摘目的建立针对高致病性2型猪链球菌（Streptococcus suis 2，SS2）89K毒力岛Ⅳ型分泌系统的环介导等温扩增（100p-mediated isothermal amplification，LAMP）方法。方法根据国内流行株特有89K毒力岛编码的Ⅳ型分泌系统（T4SS-89K）的virB4-89K基因保守区域设计并合成LAMP引物，通过优化反应体系和扩增条件建立T4SS-89K快速检测方法，对方法的特异性、敏感性进行评估。结果优化的LAMP反应体系具有良好的扩增效率，检测灵敏度为1．53×10^-1拷贝／反应，全部扩增检测可在60rain内完成；建立的LAMP法具有良好的特异性，与不含T4SS-89K的猪链球菌（包括1／2型、1型、3-33型），以及其他常见9种对照菌均不发生特异性扩增，而与1998和2005年疫情现场分离的高致病性SS2均可发生特异性扩增。结论建立的LAMP方法具有特异、灵敏，设备要求简单等特点，可用于高致病性SS2快速检测。
文摘[目的]检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV encoded small RNA-1,EBER-1)。免疫组织化学的方法检测EBER-1阳性和阴性肺癌组织中LMP-1、p53、Bcl-2及MMP-9的表达。[结果]108例肺癌组织中EBER-1阳性36例,阳性率33.3%;22例癌旁正常肺组织中EBER-1阳性1例,阳性率4.5%,差异有统计学意义(P<0.01)。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1%和4.2%,差异无统计学意义(P>0.05);在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积(AA)和积分光密度(IOD)均高于阴性组,差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9AA和IOD均高于阴性组,但差异无统计学意义(P>0.05)。[结论]EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达,进而在肺癌的发生、发展和转移中发挥作用。
