摘要
[目的]检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV encoded small RNA-1,EBER-1)。免疫组织化学的方法检测EBER-1阳性和阴性肺癌组织中LMP-1、p53、Bcl-2及MMP-9的表达。[结果]108例肺癌组织中EBER-1阳性36例,阳性率33.3%;22例癌旁正常肺组织中EBER-1阳性1例,阳性率4.5%,差异有统计学意义(P<0.01)。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1%和4.2%,差异无统计学意义(P>0.05);在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积(AA)和积分光密度(IOD)均高于阴性组,差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9AA和IOD均高于阴性组,但差异无统计学意义(P>0.05)。[结论]EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达,进而在肺癌的发生、发展和转移中发挥作用。
Purpose To investigate the expressions of Epstein-Barr virus （EBV） latent infection membrane protein 1（LMP-1）,p53,Bcl-2,matrix matalloproteinases-9（MMP-9） in tissues of lung cancer with EBV infection,and to explore their relationship with carcinogenesis of lung cancer. Methods EBV encoded small RNA-1 （EBER-1） in 108 tissue samples of lung cancer and 22 cases of cancer adjacent tissues were detected by in situ hybridization. The expressions of LMP-1,p53,Bcl-2 and MMP-9 in EBV positive and negative cases were detected immunohistochemically. Results Positive expression of EBER-1 was found in 36 of 108 cases with lung cancer （33.3%）, and in 1 of 22 cases of cancer adjacent tissues（4.5%）,with significant difference（P0.01）. Positive rate of LMP-1 in EBER-1 positive lung cancer tissues and EBER-1 negative tissues was 11.1% and 4.2% respectively,without significant difference. Average area （AA） and integral optical density（IOD） of p53 and Bcl-2 in EBER-1 positive cases were higher than those in negative tissues,with significant difference. AA and IOD of MMP-9 in EBER-1 positive tissues were higher than those in negative tissues,without significant difference（P0.05）. Conclusion EBV might play a role in carcinogenesis and metastasis of lung cancer by influencing the expressions of LMP-1,p53,Bcl-2 and MMP-9.
出处
《肿瘤学杂志》
CAS
2013年第6期431-434,共4页
Journal of Chinese Oncology
基金
河北省自然科学基金资助项目(H2013209180)
唐山市科技计划项目(121302136)
