Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship ...Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship between Rh blood group antibody and hemolytic disease of the newborn(HDN)in second-child pregnant women,and to provide laboratory basis for the diagnosis and treatment of hemolytic disease of the newborn(Rh-HDN).Methods:500 pregnant women with second child were collected as the study group and 500 pregnant women with first pregnancy as the control group(all pregnant women underwent obstetric examination in the integrated obsteric clinic of our hospital from January 2020 to January 2021).To detectethe Rh blood group antigens(D,C,c,E,e)of the two groups of samples,screene the irregular antibodies,identify the specificity of irregular antibodies,determine the titer and record the hemolytic disease of the newborn of pregnant women with positive Rh blood group antibodies.Results:There were 11 Rh phenotypes in the pregnant women with second child in the study group:CCDee(152cases,30.4%),CcDEe(136cases,27.2%)CcDee(84cases,16.8%),ccDEE(30cases,6%),ccDee(31cases,6.2%),CCDEe(14cases,2.8%),ccDEe(9cases,1.8%),cc dee(18cases,3.6%),CCDEE(2cases,0.4%),CcdEe(12cases,2.4%),Ccdee(6cases,1.2%),CCd ee(6cases,1.2%).A total of 42 cases(8.4%)in the pregnant women with second child were negative for RhD.There were 10 Rh phenotypes in the pregnant women with first pregnancy in the control group:CCDee(144cases,28.8%),CcDEe(138cases,27.6%),CcDee(90cases,18%),ccDEE(42cases,8.4%),ccDee(28cases,5.6%),CCDEe(10cases,2%),ccDEe(8cases,1.6%),cc dee(19cases,3.8%),CCDEE(1cases,0.2%),CcdEe(11cases,2.2%),Ccdee(9cases,1.8%).A total of 39 cases(7.8%)in the pregnant women with first pregnancy were negative for RhD.In the pregnant women with second child in the study group,the positive rate of irregular antibody screening was 4.0%(20/500),and the specificity of Rh blood group antibodies was found as follows:anti-E 1.8%(9/500),anti-D 1.4%(7/500),anti-C 0.4%(2/500)and anti-Ec 0.4%(2/500).The positive rate of irregular antibody screening in the pregnant women with first pregnancy in the control group was 0,and the difference between the two groups was statistically significant(P<0.05).Rh-HDN was found in 10 newborns(2%)of the 20 women with positive irregular antibodies in the pregnant women with second child,and the antibody titer during pregnancy was more than 32.No Rh-HDN occurred in newborns in the pregnant women with first pregnancy,and the difference between the two groups was statistically significant(P<0.05).Conclusion:Pregnancy stimulation can increase the probability of irregular antibodies in pregnant women,and irregular antibodies in Rh blood group can easily cause Rh-HDN,so attention should be paid to routine detection of five antigens of Rh blood group and irregular antibody screening during prenatal examination.It is helpful for the early detection of Rh-blood irregular antibodies and the assessment of fetal or neonatal risk of Rh-HDN.展开更多
Rh blood group and blood transfusion has been a hot topic in clinical research in recent years. Rh blood group incompatibility can cause hemolytic transfusion reaction and neonatal hemolytic disease (HDN). Infusion wi...Rh blood group and blood transfusion has been a hot topic in clinical research in recent years. Rh blood group incompatibility can cause hemolytic transfusion reaction and neonatal hemolytic disease (HDN). Infusion with Rh blood type or compatibility can reduce the adverse reactions of blood transfusion, avoid the production of irregular antibodies in the Rh system, quickly improve the ability to solve difficult blood matching, and improve the safety of blood transfusion therapy, which is of great clinical significance.展开更多
目的总结1例Rh系统抗-c胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)的实验室检测并进行文献复习,探究抗-c HDFN的特点。方法采用血清学方法检测患儿及其母亲的ABO血型、Rh分型、直接抗人球蛋白试验(DAT)、不规...目的总结1例Rh系统抗-c胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)的实验室检测并进行文献复习,探究抗-c HDFN的特点。方法采用血清学方法检测患儿及其母亲的ABO血型、Rh分型、直接抗人球蛋白试验(DAT)、不规则抗体及其效价,并对本实验室及国内外抗-c HDFN案例进行统计,分析比较抗-c与抗-D、抗-E引起HDFN的严重率。结果患儿血型为B、CcDee,DAT阳性,血清和放散液均检测出抗-c,血清抗体效价为4;母亲血型为AB、CCDee,DAT阴性,血清中检测出抗-c,效价为128。20例抗-c HDFN案例中DAT阳性有17例,输血或换血的有9例,占45%(9/20)。严重率为:抗-c 47.60%(10/21)、抗-D 47.60%(10/21)、抗-E 31.30%(5/16)。结论母亲妊娠和/或输血是产生抗-c等Rh系统同种抗体的主要原因。抗-c的预防管理应类同抗-D,对育龄女性输血要做好Rh血型5种抗原匹配输注以避免抗体产生,并重视孕妇产检Rh血型鉴定和抗体筛查鉴定,做到早发现、早干预、早治疗。展开更多
基金Youth Fund Program of Hainan Provincial Natural Science Fundation of China(No.820QN410)。
文摘Objective:To investigate the phenotype distribution of five antigens of Rh blood group system and the specificity of Rh blood group irregular antibodies in pregnant women with second child.To analyze the relationship between Rh blood group antibody and hemolytic disease of the newborn(HDN)in second-child pregnant women,and to provide laboratory basis for the diagnosis and treatment of hemolytic disease of the newborn(Rh-HDN).Methods:500 pregnant women with second child were collected as the study group and 500 pregnant women with first pregnancy as the control group(all pregnant women underwent obstetric examination in the integrated obsteric clinic of our hospital from January 2020 to January 2021).To detectethe Rh blood group antigens(D,C,c,E,e)of the two groups of samples,screene the irregular antibodies,identify the specificity of irregular antibodies,determine the titer and record the hemolytic disease of the newborn of pregnant women with positive Rh blood group antibodies.Results:There were 11 Rh phenotypes in the pregnant women with second child in the study group:CCDee(152cases,30.4%),CcDEe(136cases,27.2%)CcDee(84cases,16.8%),ccDEE(30cases,6%),ccDee(31cases,6.2%),CCDEe(14cases,2.8%),ccDEe(9cases,1.8%),cc dee(18cases,3.6%),CCDEE(2cases,0.4%),CcdEe(12cases,2.4%),Ccdee(6cases,1.2%),CCd ee(6cases,1.2%).A total of 42 cases(8.4%)in the pregnant women with second child were negative for RhD.There were 10 Rh phenotypes in the pregnant women with first pregnancy in the control group:CCDee(144cases,28.8%),CcDEe(138cases,27.6%),CcDee(90cases,18%),ccDEE(42cases,8.4%),ccDee(28cases,5.6%),CCDEe(10cases,2%),ccDEe(8cases,1.6%),cc dee(19cases,3.8%),CCDEE(1cases,0.2%),CcdEe(11cases,2.2%),Ccdee(9cases,1.8%).A total of 39 cases(7.8%)in the pregnant women with first pregnancy were negative for RhD.In the pregnant women with second child in the study group,the positive rate of irregular antibody screening was 4.0%(20/500),and the specificity of Rh blood group antibodies was found as follows:anti-E 1.8%(9/500),anti-D 1.4%(7/500),anti-C 0.4%(2/500)and anti-Ec 0.4%(2/500).The positive rate of irregular antibody screening in the pregnant women with first pregnancy in the control group was 0,and the difference between the two groups was statistically significant(P<0.05).Rh-HDN was found in 10 newborns(2%)of the 20 women with positive irregular antibodies in the pregnant women with second child,and the antibody titer during pregnancy was more than 32.No Rh-HDN occurred in newborns in the pregnant women with first pregnancy,and the difference between the two groups was statistically significant(P<0.05).Conclusion:Pregnancy stimulation can increase the probability of irregular antibodies in pregnant women,and irregular antibodies in Rh blood group can easily cause Rh-HDN,so attention should be paid to routine detection of five antigens of Rh blood group and irregular antibody screening during prenatal examination.It is helpful for the early detection of Rh-blood irregular antibodies and the assessment of fetal or neonatal risk of Rh-HDN.
文摘Rh blood group and blood transfusion has been a hot topic in clinical research in recent years. Rh blood group incompatibility can cause hemolytic transfusion reaction and neonatal hemolytic disease (HDN). Infusion with Rh blood type or compatibility can reduce the adverse reactions of blood transfusion, avoid the production of irregular antibodies in the Rh system, quickly improve the ability to solve difficult blood matching, and improve the safety of blood transfusion therapy, which is of great clinical significance.
文摘目的总结1例Rh系统抗-c胎儿新生儿溶血病(hemolytic disease of the fetus and newborn,HDFN)的实验室检测并进行文献复习,探究抗-c HDFN的特点。方法采用血清学方法检测患儿及其母亲的ABO血型、Rh分型、直接抗人球蛋白试验(DAT)、不规则抗体及其效价,并对本实验室及国内外抗-c HDFN案例进行统计,分析比较抗-c与抗-D、抗-E引起HDFN的严重率。结果患儿血型为B、CcDee,DAT阳性,血清和放散液均检测出抗-c,血清抗体效价为4;母亲血型为AB、CCDee,DAT阴性,血清中检测出抗-c,效价为128。20例抗-c HDFN案例中DAT阳性有17例,输血或换血的有9例,占45%(9/20)。严重率为:抗-c 47.60%(10/21)、抗-D 47.60%(10/21)、抗-E 31.30%(5/16)。结论母亲妊娠和/或输血是产生抗-c等Rh系统同种抗体的主要原因。抗-c的预防管理应类同抗-D,对育龄女性输血要做好Rh血型5种抗原匹配输注以避免抗体产生,并重视孕妇产检Rh血型鉴定和抗体筛查鉴定,做到早发现、早干预、早治疗。
