摘要
目的探讨毛细管超速离心法对患者RhCcEe血型抗原混合视野鉴定的应用价值。方法选取2020年12月—2024年4月在我院就诊的RhCcEe抗原呈混合视野的患者1155例,经毛细管超速离心法分离自身红细胞并鉴定Rh血型,并回顾性分析受、供者RhCcEe抗原相容情况。结果毛细管超速离心技术能成功分离995例(86.15%)患者自身红细胞并成功鉴定Rh血型,Rh血型分布情况CCDee>CcDEe>ccDEE>CcDee。受、供者RhCcEe抗原不相容性输注比例较高(35.41%)。当受血者接受来自多位供血者的血液输注时,其发生Rh抗原不相容性输血反应的风险显著增加(P<0.001)。结论毛细管超速离心法分离自身红细胞有助于RhCcEe抗原混合视野的患者Rh血型的鉴定,为RhCcEe配合性输血提供基础。
Objective To explore the value of capillary ultracentrifugation for RhCcEe phenotype identification in patients with mixedfield agglutination reaction(MF).Methods A total of 1155 patients with MF in RhCcEe phenotype identification from December 2020 to April 2024 were enrolled at First Affiliated Hospital of Nanjing Medical University.The young erythrocytes were separated by capillary ultracentrifugation and identified the RhCcEe antigen,then retrospectively analyzed the RhCcEe antigen compatibility of recipients and donors.Results The distribution of Rh blood groups in 995(86.15%)patients successfully identified by capillary ultracentrifugation was CCDee>CcDEe>ccDEE>CcDee.The proportion of RhCcEe antigen incompatibility transfusion was high(35.41%).When recipients received blood transfusions from multiple donors,their risk of Rh antigen incompatibility transfusion reactions was significantly increased(P<0.001).Conclusion Capillary ultracentrifugation was helpful for identifying Rh blood groups in patients with MF of RhCcEe antigens and provided the basis for compatible blood transfusion.
作者
宋志群
李莺
朱雯雯
孙小红
周小玉
SONG Zhiqun;LI Ying;ZHU Wenwen;SUN Xiaohong;ZHOU Xiaoyu(Department of Blood Transfusion,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029)
出处
《临床输血与检验》
2025年第6期796-801,共6页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
毛细管超速离心法
RH血型鉴定
配合性输注
Capillary ultracentrifugation
Rh blood group identification
Compatible blood transfusion
