N6-methyladenosine(m6A)plays a key role in mammalian early embryonic development and cell lineage differentiation.However,the role and mechanisms of 18S ribosomal RNA(rRNA)m6A methyltransferase METTL5 in early embryon...N6-methyladenosine(m6A)plays a key role in mammalian early embryonic development and cell lineage differentiation.However,the role and mechanisms of 18S ribosomal RNA(rRNA)m6A methyltransferase METTL5 in early embryonic development remain unclear.Here,we found that 18S rRNA m6A methyltransferase METTL5 plays an important role in porcine early embryonic development.METTL5 knockdown and overexpression significantly reduced the developmental efficiency of porcine early embryos and impaired cell lineage allocation.METTL5 knockdown apparently decreased the global translation efficiency in blastocyst,while METTL5 overexpression increased the global translation efficiency.Furthermore,METTL5 knockdown did not affect the abundance of CDX2 mRNA,but resulted in a significant reduction in CDX2 protein levels.Moreover,the low developmental efficiency and abnormal lineage distribution of METTL5 knockdown embryos could be rescued by CDX2 overexpression.Collectively,our results demonstrated that 18S rRNA methyltransferase METTL5 regulates porcine early embryonic development via modulating the translation of CDX2.展开更多
基金supported by grants from the Sub-project of National Key Research and Development Program of China(2021YFA0805905-1)the Special Fund for Anhui Agriculture Research System,China(AHCYJSTX-04)+2 种基金the Joint Research Project on the Anhui Local Pigs Breeding and Utilization,China(340000211260001000431)the Open Project of Key Laboratory of Embryo Development and Reproductive Regulation of Anhui Province,China(FSKFKT004)the Major Special Science And Technology Project of Anhui Province,China(202103a06020013)。
文摘N6-methyladenosine(m6A)plays a key role in mammalian early embryonic development and cell lineage differentiation.However,the role and mechanisms of 18S ribosomal RNA(rRNA)m6A methyltransferase METTL5 in early embryonic development remain unclear.Here,we found that 18S rRNA m6A methyltransferase METTL5 plays an important role in porcine early embryonic development.METTL5 knockdown and overexpression significantly reduced the developmental efficiency of porcine early embryos and impaired cell lineage allocation.METTL5 knockdown apparently decreased the global translation efficiency in blastocyst,while METTL5 overexpression increased the global translation efficiency.Furthermore,METTL5 knockdown did not affect the abundance of CDX2 mRNA,but resulted in a significant reduction in CDX2 protein levels.Moreover,the low developmental efficiency and abnormal lineage distribution of METTL5 knockdown embryos could be rescued by CDX2 overexpression.Collectively,our results demonstrated that 18S rRNA methyltransferase METTL5 regulates porcine early embryonic development via modulating the translation of CDX2.
