摘要
背景与目的:肠化生被认为是胃癌的癌前病变,而肠特异性转录因子CDX2在肠上皮的形成、分化及肠表型的维持方面有重要作用。近年来研究发现CDX2在慢性萎缩性胃炎(chronicatrophicgastritis,CAG)相关的肠化生中呈高水平表达,在部分胃癌组织中也有表达,提示其可能与胃粘膜上皮由胃表型向肠表型的转化,以及胃癌的发生有关。本研究旨在探讨CDX2在胃粘膜肠化生发生、进展及胃癌发生中的作用,进一步明确肠化生与胃癌发生的关系。方法:选择46例CAG伴肠化生、40例胃癌及32例对应癌旁肠化生,构建组织芯片。分别用高铁二铵/爱先蓝(HID/AB)及HE染色对肠化生及胃癌进行分型,然后用免疫组化和原位杂交检测不同亚型肠化生及胃癌中CDX2蛋白及mRNA的表达。结果:癌旁肠化生中Ⅲ型肠化生的比例显著高于CAG伴肠化生(分别为56.25%和21.74%,P<0.01)。CDX2蛋白阳性率在CAG伴肠化生、癌旁肠化生和胃癌中分别为69.56%、53.13%和42.50%;CDX2mRNA阳性率分别为63.04%、46.87%和35.00%。胃癌中显著低于CAG伴肠化生(P<0.01),而与癌旁肠化生无显著性差异(P>0.05)。CDX2表达与胃癌组织类型有关联,肠型胃癌显著高于弥漫型(P<0.05)。Ⅲ型肠化生中CDX2蛋白表达显著低于Ⅰ型(P<0.05)。结论:CDX2在胃粘膜肠化生发生及进展为胃癌过程中可能有重要作用。
BACKGROUD ＆ OBJECTIVE; Intestinal metaplasia （IM） is thought as the precancerous lesion of gastric carcinoma, and CDX2 gene plays important roles in development and differentiation of intestinal epithelium, and maintenance of intestinal phenotype. Recent studies found that CDX2 were expressed aberrantly in IM of chronic atrophic gastritis （CAG） and some gastric carcinomas, which implied that CDX2 may play an important role in IM formation and gastric carcinogenesis, This study was to investigate the roles of CDX2 in the development and progression of IM and gastric carcinogenesis, and determine the correlation of IM to gastric carcinogenesis. METHODS. A tissue microarray containing 46 cases of CAG with IM, 40 cases of gastric carcinoma, and 32 cases of IM foci in paracancerous tissues was constructed, High iron diamine/alcian blue （HID/ AB） and HE staining were used to classify IM and gastric carcinoma, and the expression of CDX2 protein and mRNA in different gastric lesions was assessed with immunohistochemistry and in situ hybridization, respectively. RESULTS. The proportion of type III IM was significantly higher in IM foci in paracancerous tissues than in CAG with IM （56.25% vs. 21.74%, P〈0,01）, The positive rates of CDX2 protein were 69.56% in IM foci in CAG, 53,13% in IM foci in paracancerous tissues, and 42.50% in gastric carcinomas, and the positive rates of CDX2 mRNA were 63.04%, 46.87%, and 35.00%, respectively, The positive rates were significantly lower in gastric cancer than in IM in CAG （P〈0,01）, but there was no significant difference between gastric cancer and IM foci in paracancerous tissues （P〉0.05）. The expression of CDX2 protein and mRNA was significantly higher in intestinal-type gastric cancer than in diffuse-type gastric cancer （54.55% vs, 27.78%, 45.45% vs, 22.22%, P〈0.05）, The expression of CDX2 protein was significantly lower in type Ⅲ IM than in type Ⅰ IM （46.42% vs. 79.31%, P〈0.05）. CONCLUSIONS. CDX2 may play important roles in the development and progression of IM and gastric carcinogenesis,
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2006年第2期185-189,共5页
Chinese Journal of Cancer
基金
卫生部临床学科重点项目(No.20012130)~~
关键词
胃肿瘤/病因学
CDX2
肠化生
组织芯片
原位杂交
Gastric neoplasms/etiology
CDX2
Intestinal metaplasia Tissue microarray
In situ hybridization
