To investigate the HBV quasispecies population in the patients with chronic HBV infection,a set of specific primers was synthesized according to DNA sequence of HBV found in China The reverse transcriptase(RT) region ...To investigate the HBV quasispecies population in the patients with chronic HBV infection,a set of specific primers was synthesized according to DNA sequence of HBV found in China The reverse transcriptase(RT) region in polymerase gene was amplified by PCR method from the sera of 3 patients with chronic HBV infection,and then the PCR products were subcloned into pGEM Teasy vectors 13 clones were sequenced Sequence comparison of the selected clones was made to find out the differences After being compared,11 sequences of RT were found different The different rates of RT coding nucletide sequences, RT and HBsAg amino acid sequences of the 11 clones were 5 1%,4 9% and 7 5% respectively Many mutation types,including point substitution and deletion mutation,were found in this region There are quasispecies population and defective HBV genome in patients with chronic HBV infection.展开更多
文摘To investigate the HBV quasispecies population in the patients with chronic HBV infection,a set of specific primers was synthesized according to DNA sequence of HBV found in China The reverse transcriptase(RT) region in polymerase gene was amplified by PCR method from the sera of 3 patients with chronic HBV infection,and then the PCR products were subcloned into pGEM Teasy vectors 13 clones were sequenced Sequence comparison of the selected clones was made to find out the differences After being compared,11 sequences of RT were found different The different rates of RT coding nucletide sequences, RT and HBsAg amino acid sequences of the 11 clones were 5 1%,4 9% and 7 5% respectively Many mutation types,including point substitution and deletion mutation,were found in this region There are quasispecies population and defective HBV genome in patients with chronic HBV infection.
