摘要
探讨乙型肝炎病毒(HBV)基因组异质性及其生物学意义。以已知HBV基因序列为依据 ,设计特异性多聚酶链反应 (PCR)引物 ,自 2例慢性HBV感染患者外周血血清中扩增HBV基因组序列 ,克隆入pGEMTeasy质粒 ,随机挑选克隆进行DNA测序并加以比较。测序结果发现 ,来源不同的 5株HBV全基因组核苷酸序列的一致率为 93 6 % ,不同克隆的 4个开放读码框架长度有明显区别 ,氨基酸序列中存有移框、缺失、替换等多种变异类型。说明来源于同一患者的HBV基因组序列之间存在有较明显的变异现象 ,在患者体内构成了准种群。
To investigate the HBV quasispecies groups in the patients with chronic HBV infection. A set of specific primers was synthesized according to HBV DNA sequence, the whole genome was amplified by PCR method from the serum of 2 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. Five clones were randomly selected to be sequenced. Sequence comparison showed that each sequence of selected clones was distinctly different, and the nucleic acid identity rate of these 5 clones was 93 6%. Substitution, deletion and frame shift were found in the amino acid sequences of 4 ORFs from the 5 clones. The sequencing comparison implies that there were HBV quasispecies groups in chronically infected patients.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第2期116-118,共3页
Medical Journal of Chinese People's Liberation Army
