摘要
根据大鼠肝再生增强因子 (Augmenterofliverregeneration ,ALR)cDNA序列设计特异性引物 ,采用多聚酶链反应 (PCR)法自Wistar大鼠基因组DNA中扩增出大鼠ALR基因组DNA ,全长为 15 0 8nbp ,结构为 3个外显子和 2个内含子 ,外显子长度分别为 18bp、197bp和 16 3bp ,各编码 6个、6 4个和 5 5个氨基酸残基 ,长度与小鼠、人类ALR的外显子一致。
WT5”BZ]To investigate the structure of the genomic DNA sequence for ALR,specific PCR method was used to sub clone target fragment from the Wistar rat genome The whole length is 1508bp,including 3 exons and 2 introns The exons are 18bp,197bp and 163bp,each one encode 6aa,64aa and 55aa respectively The lengths of the exon are as long as that of human and mouse [WT5”HZ]
出处
《临床肝胆病杂志》
CAS
北大核心
2001年第1期36-37,共2页
Journal of Clinical Hepatology
