摘要
研究了用固相萃取预分离,微柱高效液相色谱法测定茶叶中多酚的方法。茶叶中的多酚用80%乙醇超声震荡萃取,提取液用C18固相萃取小柱预分离,以WatersXterraTMRP18(1.0×50mm,2.5μm)色谱为固定相,1%的醋酸和甲醇梯度洗脱为流动相,在该条件下茶叶中主要的多酚在3.0min内可达到基线分离;用紫外二极管矩阵检测器检测,方法标准回收率为95%～103%,相对标准偏差为1.2%～1.6%。用该方法测定了几种茶叶样品中的多酚,结果令人满意。
A microcolumn high performance liquid chromatography method for the determination of tea polyphenols wasstudied. Tea polyphenols was extracted from the tea leaf with 80% of ethanol by ultrasonic extraction at 50℃, then purified bysolid phase extraction with Sep-Pak-C18 cartridge. The tea polyphenols can be separated on a Waters XterraTM RP18 (1.0×50mm,2.5μm) microcolumn, with methanol and 1% acetic acid solution gradient elution as mobile phase at a flow-rate of 0.2ml/min, andmonitored with photodiode array detector at 200～400nm. The recovery of tea polyphenols is 95%～103%, and relative standarddeviation is 1.22%～1.6%. This method can be applied to the determination of tea polyphenols in tea with good result.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第6期147-149,共3页
Food Science
基金
云南省教育厅资助项目(011143)
关键词
微柱高效液相色谱法
测定
茶多酚
茶叶
high performance liquid chromatography
tea polyphenols
tea leaf
