摘要
建立了柑橘中2，4-二氯苯氧乙酸（2，4-D）残留的高效液相色谱（HPLC）测定方法。样品用水提取，提取液经二氯甲烷萃取、浓缩后，用丙酮溶解。丙酮溶液上硅胶固相萃取柱，用甲醇洗脱，洗脱液氮气吹干后用流动相溶解。HPLC条件：二极管矩阵检测器检测；波长：282nm；色谱柱：ODS-3 C18柱（4．6mm×250mm，5μm）；流动相：甲醇-水为8：2（体积比），pH3．0。在0．2～2．0mg／kg添加范围内，回收率86．5％～96．6％，变异系数2．45％～8．35％，最低检测限为0．05mg／kg。
A high performance liquid chromatographic method was developed for detecting 2,4-dichlorophenoxyacetic acid 2,4-D in orange. 2,4-dichlorophenoxyacetic acid was extracted from orange with water, purified by passing through silica solid-phase extraction cartridge. For analysis, a ODS-3 C18 colunm （4. 6 mm × 150 mm, 5 μm） was used at 30 ℃ and fluted with methanol-water （80 ： 20, V/V, pH 3.0） at flow rate of 1.0 ml/min, and the detection wavelength was 282 nm. Average recoveries of 2,4-dichlorophenoxyacetic acid from the oranges fortified at a level of 0. 2 -2. 0 mg/kg were 86. 5% -96. 6%, with the CVs of 2. 45% - 8.35%. The detection limit for 2,4-dichlorophenoxyacetic acid was 0. 05 mg/kg.
出处
《江苏农业学报》
CSCD
北大核心
2007年第1期67-70,共4页
Jiangsu Journal of Agricultural Sciences
基金
江苏省地方标准项目(028045510508)
关键词
高效液相色谱
固相萃取
2
4-二氯苯氧乙酸(2
4-D)
柑橘
high performance liquid chromatography
solid-phase extraction
2,4-dichlorophenoxyacetic acid （2, 4-D）
orange
