摘要
以45SrDNA为探针,获得了草棉体、亚洲棉体细胞染色体的荧光原位杂交(FISH)资料。从rDNA FISH实验结果看,草棉有6个杂交信号,也显示了3对核仁组织区(NOR),分别位于第3、9、13对同源染色体上;亚洲棉有4个杂交信号,显示了2对NOR,分别位于第6、13对同源染色体上。草棉、亚洲棉基于45SrDAN FISH的核型分别为:2n=2x=26=20m(4sat)+6sm(2sat)和2n=2x=26=26m(4sat)。
Based on the fluorescein in situ hybridization (FISH) of somatic chromosome of G. herbaceum L., and G. arboreum L., with the probe of 45S rDNA, The FISH results revealed that there were six hybridized signals on chromosomes of G.herbaceum L., showed three pairs of NOR(Nuclear Organization Region) which were located in chromosome number 3,9 and 13, repectively. There were four hybridized signals on chromosomes of G.arboreum L.,showed two pairs of NOR, which were located in chromosome number 6 and 13,respectively. The karyotype formula of G.herbaceum L., based on its 45S rDAN-FISH was 2n=2x=26=20m (4sat)+6sm(2sat)和2n=2x=26=26m(4sat).
出处
《棉花学报》
CSCD
北大核心
2004年第4期223-228,共6页
Cotton Science
基金
国家自然科学基金
863计划
