摘要
【目的】为了探明普通西瓜种(Citrullus lanatus)3个变种间的亲缘关系和进化与驯化过程,【方法】利用双色荧光原位杂交技术(fluorescence in situ hybridization,FISH),将核糖体45S rDNA和5S rDNA在11份栽培变种(Citrulluslanatus subsp.vulgaris)、2份Egusi变种(C.lanatus subsp.mucosospermus)和3份Citroides变种(C.lanatus subsp.lana-tus)有丝分裂中期染色体上进行了定位研究。11份栽培变种西瓜和2份Egusi类型西瓜都含有2对45S rDNA位点和1对5S rDNA位点,3份Citroides变种都含有1对45S rDNA位点和2对5S rDNA位点。【结果】结果表明,普通西瓜种3个变种内部各基因型间具有相同的45S rDNA和5S rDNA分布模式,栽培变种和Egusi变种具有相同的分布模式,但它们均与Citroides变种存在明显差异,说明栽培变种与Egusi变种之间的亲缘关系更近一些。【结论】rDNA序列分布分析为在分子细胞学水平开展栽培西瓜的进化与驯化分析提供了证据。
[Objective] The objective of the study is to investigate the genetic relationship ,the process of evolution and domestication in watermelon. [Method]Both 45S rDNA and 5S rDNA were used as probes in the double fluorescence in situ hybridization （FISH） to be localized on chromosomes in 16 genotypes rep- resenting three subspecies. There are two pairs of 45S rDNA loci and one pair 5S rDNA locus in C. lanatus subsp. Vu/gar/s and subsp. Mucosospermus. C. lanatus subsp, lanatus possess one pair 45S rDNA locus and two pairs of 5S rDNA loci. [Result] The result showed that genotypes belonging to one subspecies have similar rDNA distribution pattern, and there was no significant differences between C.lanatus subsp. Vulgaris and C. lanatus subsp. Mucosospermus. C. lanatus subsp, lanatus is quiet different from the other two subspecies in the number and position of rDNAs sites. It is suggested that the distance between C. lanatus subsp. Vulgaris and C. lanatus subsp. Mucosospermus are closer than that of C. lanatus subsp. Lanatus. [Conclusion]The distribution analysis of rDNA sequences provides evidence in evolution and domestication for cultivated watermelon in molecular biology level.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第5期764-769,I0004,共7页
Journal of Fruit Science
基金
科技部国际合作项目(2010DFB33740)
国家现代西甜瓜产业技术体系(CARS-26)
国家自然科学基金项目(31171980)
北京市自然科学基金重大项目(5100001)
育种平台三期(D111100001311002)
