摘要
采用砂培试验,用不同浓度的NaCl和等渗PEG6000(聚乙二醇6000,渗透势约为-0.44MPa)处理生长20d的菊芋幼苗,3d后分别测定其根、茎、叶中的Na+、K+、Cl-含量以及叶片SOD、POD活性。结果表明,在NaCl和PEG胁迫下,根、茎、叶的Na+、Cl-含量不断升高,而K+含量保持稳定。其中,茎中Na+含量高于根和叶。NaCl胁迫下,根、茎、叶的SK、Na值随胁迫强度的增加而递增,茎中SK、Na值小于根和叶。随着NaCl胁迫强度的增加,菊芋幼苗叶片的SOD和POD活性先上升后下降;PEG处理下,SOD活性分别高于对照和等渗NaCl处理31.1%和27.1%;而POD活性却分别低于对照和等渗NaCl处理26.0%和36.1%。
In this sand culture experiment,Helianthus tuberosus L.seedlings of 20 days were treated with different concentrations of NaCl and iso-osmotic PEG solutions (osmotic potential is about -0.44 MPa).The content of Na^+,K^+ and Cl^- in root,stem and leaf,the activities of SOD and POD in leaf were determined in the third day after treatment.The results showed that under NaCl and PEG stresses,the content of Na^+,Cl^- in root,stem and leaf increased but the content of K^+ had no significant changes.The content of Na^+ in stem is higher than that in root and leaf.With the concentration of NaCl increasing,the selective adsorption of K^+ and Na^+ (S_(K,Na)) for root,stem and leaf gradually increased,and S_(K,Na) for stem was lower than that for root and leaf.With the concentration of NaCl increasing,activities of SOD and POD in leaf increased at first then decreased.As compared with under condition of control treatment and iso-osmotic (NaCl) stress,under PEG stress,SOD activities in leaf increased 31.1% and 27.1%,but POD activities decreased 26.0% and 36.1% respectivlly.
出处
《西北植物学报》
CAS
CSCD
2004年第7期1241-1245,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家863计划项目(2002AA2Z4061)
关键词
菊芋
盐胁迫
水分胁迫
离子
SOD
POD
Helianthus tuberosus L.
salt stress
water stress
ion
SOD
POD
