摘要
目的探查胰腺丝氨酸弹力蛋白酶mRNA在大鼠组织器官中的表达。方法克隆地高辛素标记的大鼠胰腺丝氨酸弹力蛋白酶Ⅱ的RNA探针,摘取Wistar雄性正常大鼠的组织器官,固定于4%多聚甲醛,进行原位杂交。结果正常大鼠腹主动脉的内皮细胞、中膜平滑肌细胞以及血管外膜成纤维细胞,血液细胞中的淋巴细胞、单核细胞,气管透明软骨细胞,胰腺的腺细胞、腮腺、颔下腺上皮细胞,肝细胞、肝窦壁的内皮细胞,小肠黏膜杯状细胞,心肌细胞,肾间质的纤维母细胞,肺泡上皮细胞,大脑胶质细胞,皮肤真皮纤维母细胞,睾丸曲精细管内的初级精母细胞、次级精母细胞以及精子,脾脏以及胸腺的淋巴细胞等,均有弹力蛋白酶mRNA的表达。结论胰腺丝氨酸弹力蛋白酶mRNA广泛表达于正常大鼠血管,气管,胰腺,肝,小肠,心,肾,脑,皮肤,睾丸,脾,胸腺等组织器官中。
Objective:To study expression of the normal rats' tissues and organs by use pancreas serine elastase mRNA.Methods:Rat pancreas serine elastaseⅡRNA probe was cloned and labeled with digoxin.The tissues and organs of normal male Wistar rats were taken and fixed in4%paraformaldehyde.In situ hy-bridization was then performed.Results:Expression of elastase mRNA has been found in the endothelial cells,the medial smooth muscle cells and the adventitial fibroblasts of the abdominal aorta,the lymphocytes,monocytes in blood,the tracheal hyaline cartilaginous cells,the glandular cells of the pancreas,the epithelial cells of the parotid gland and submaxillary gland,the hepatocytes,the endothelial cells of the liver sinusoid wall,the goblet cells of the mucous membrane of the small intestine,the cardiac myocytes,the renal intersti-tial fibroblasts,the alveolar epithelial cells,the cerebral glial cells,the fibroblasts of the dermis corium of the skin,the primary spermaocytes,the secondary spermaocytes and sperm in the seminiferous tubule of the testis,the lymphocytes in the spleen and thymus.Conclusions:Elastase mRNA expressed extensively in the tissues and organs of rats described above.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第11期44-48,共5页
China Journal of Modern Medicine
