摘要
目的 :采用ECV30 4细胞 (人脐静脉内皮细胞株 )构建体外血管新生的三维模型 ,为ECV30 4细胞应用于体外血管新生的研究提供依据。方法 :制作鼠尾胶原三维凝胶 ,在凝胶表面种植ECV30 4细胞 ,培养至接近汇合状态 ,换用含碱性成纤维细胞生长因子 (bFGF)的培养液继续培养 3～ 12d ,用倒置相差显微镜观察内皮细胞株向凝胶内迁移、生长状况。结果 :bFGF组ECV30 4细胞迁移到凝胶内不同层次 ,形成细胞条索并吻合成网 ;垂直切面上可见ECV30 4细胞从凝胶表面的单细胞层向凝胶内发芽生长 ,形成树枝状毛细血管样结构。结论 :ECV30 4细胞在三维胶原凝胶基质培养模型中 ,在适当的因子诱导下 ,能够表现出血管新生中的增殖、迁移、发芽等步骤 ,可以作为内皮工具来研究体外血管新生。
Objective:To develop an experimental three-dimensional model by ECV304 cells(human umbilical vein endothelial cell line) for investigating the mechanisms of angiogenesis in vitro.Methods:ECV304 cells were seeded onto three-dimensional collagen gels made of rat-tail collagen.When the endothelial cells were cultured and grown to near confluence,treated with bFGF for 3 to 12 days,and then assessed with inverted phase contrast microscope.Results:The endothelial cells migrated into the gels,formed complex networks by cell cords at different levels through the bottom view,and sprouted capillary-like structures through the side view.Conclusion:ECV304 cells are capable of expressing some early events of angiogenesis in the three-dimensional collagen gels:proliferating,migrating and sprouting and so on.It should be useful for studying angiogenesis in vitro.
出处
《中国现代普通外科进展》
CAS
2004年第3期165-167,共3页
Chinese Journal of Current Advances in General Surgery
基金
山东省自然科学基金资助项目 (Y2 0 0 0C12 )
