摘要
目的探讨重组人肝细胞生长因子(rhG-HGF)联合成纤维细胞生长因子(bFGF)对下肢缺血动物模型血管新生的影响。方法制作80只小鼠左下肢缺血模型,术后随机分为4组,每组20只:(1)生理盐水对照组;(2)bFGF组;(3)rhG-HGF组;(4)rhG-HGF+bFGF组。应用多谱勒超声血流测定、肌肉毛细血管密度测定,比较4组缺血肢体血流/正常肢体血流比值及肌肉毛细血管密度。结果术后4周,4组缺血肢体血流/正常肢体血流比值及肌肉毛细血管密度为:rhG-HGF+bFGF组>rhG-HGF组>bFGF组>生理盐水组(均P<0.05)。结论rhGvHGF促进血管新生作用强于bFGF,两者联合应用有协同作用,可以更明显改善下肢缺血状况。
Objective To study the effect of combination of recombinant human hepatocyte growth factor （rhG-HGF） and fibroblast growth factor （bFGF） on angiogenesis in limb ischemic mouse model. Methods Eighty unilateral ischemic lower extremity mouse models established by operation were randomly divided into four groups ： saline group （ n = 20 ） , bFGF group （ n = 20） , rhG-HGF group （ n -- 20 ） , and rhG-HGF and bFGF group; ischemic/normal limb Doppler blood flowmetry ratio and capillary density were campared, postoperatively. Results At 4 weeks after operation, the ischemic/normal limb Doppler blood flowmetry ratio and capillary density Were as follows ： rhG-HGF ＋ bFGF group 〉 rhG-HGF group 〉 bFGF group 〉 saline group （ all P 〈 0.05 ） . Conclusions The stimulatory effect of rhG-HGF on angiogenesis is stronger than that of bFGF, while the combination of rh-HGF and bFGF has a significant synergistic effect and can more markcdly improve the vascular condition in limb isehemia.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2009年第6期584-587,共4页
China Journal of General Surgery
