摘要
目的 了解在广东省受血及献血者中发现的HIV 1亚型的流行规律及其与国际参考株的同源性。方法 应用套式聚合酶链反应 (PCR)对 1 4例采自于广东省HIV 1抗体阳性的受血者或献血者淋巴细胞富集液的核酸样品进行扩增 ,并使用ABI377型测序仪对扩增产物测序后 ,对其ENV基因C2 V3段的核酸序列进行比较分析。结果 1 4份血样中 ,7份为泰国B’亚型 ,与国际参考株RL4 2的距离最近 ,基因离散率为 (6 0 82± 2 6 0 7) % ,组内离散率为 (5 96 3± 2 383) % ;4份为AE重组亚型 ,与国际参考株TH 90 CM2 4 0最近 ,基因离散率为 (8 90 0± 1 830 ) % ,组内离散率为 (1 3 81 0± 1 31 7) % ;2份为 0 7 BC重组亚型 ,与国际参考株CN 97 C5 4A最近 ,基因离散率为 (4 1 5 5± 1 2 2 3) % ,组内基因距离为 6 36 % ;1份为 0 8 BC重组亚型 ,与国际参考株 97CNGX 9F最近 ,基因距离为 0 97%。结论 本次检测的广东省受血及献血者HIV 1以泰国B’亚型为主 ,也存在主要在性途径感染人群中流行的AE重组亚型和吸毒者中流行的 0 7 BC、0 8 BC重组亚型。
Objective To determine HIV 1 subtypes in blood receivers and blood donators and to compare their homogeneity with international reference strain. Methods HIV 1 DNA from 14 blood donators and blood receivers in Guangdong were amplified by nested PCR. The C2 V3 regions of HIV 1 ENV gene from the PCR product was sequenced and analyzed. Results The 14 samples were confirmed to be infected with B'( n =7) subtype and Circulating Recombinant Forms(CRFs):AE( n =4), 07 BC( n =2) and 08 BC( n =1). Genetic distances showed that the B' group is nearest to RL42 strain isolated in Thailand. The AE group is nearest to CM240 strain isolated in Thailand. The 07 BC group is nearest to C54A strain isolated in northeastern China. The 08 BC group is nearest to 97CNGX 9F strain isolated in Guangxi province of China. Conclusion HIV 1 subtype B' was the predominant subtype in blood receivers and blood donators in Guangdong province, but CRFs AE mainly sexually transmitted and CRFs 07 BC and 08 BC mainly circulated among drug users were also found.
出处
《华南预防医学》
2004年第2期1-3,共3页
South China Journal of Preventive Medicine
基金
广东省医学科学技术研究基金 (项目编号 :WSTJJ2 0 0 112 2 64 40 10 5 1972 0 2 12 0 0 61)
