摘要
目的了解广东省珠江三角洲地区吸毒者HIV-1亚型的流行规律及其与国际参考株的同源性。方法应用套式聚合酶链反应(PCR)对43例采自广东省珠江三角洲HIV-1抗体阳性的吸毒者淋巴细胞富集液,并对其核酸样品进行扩增,使用ABI377型测序仪对扩增产物测序后,对其ENV基因C2-V3段的核酸序列进行比较分析。结果43份血样中,29份为07-BC重组亚型,与国际参考株CN.97.C54A最近,基因离散率为(2.291±1.055)%,组内离散率为(3.534±1.306)%;9份为AE重组亚型,与国际参考株01AE.TH.90.CM240最近,基因离散率为(8.068±0.534)%,组内离散率为(2.823±1.235)%;3份为08-BC重组亚型,与国际参考株97CNGX-9F最近,基因离散率为(1.403±0.681)%,组内离散率为(1.507±0.681)%;2份为泰国B(B′)亚型,与国际参考株RL42最近,基因离散率为(7.335±3.330)%,组内距离为8.52%。结论广东省珠江三角洲地区吸毒者感染的HIV-1以曾经主要在西北地区吸毒人群中流行的07-BC重组亚型为主,也存在主要以性传播途径感染的人群中流行的AE重组亚型,提示珠江三角洲地区吸毒者中流行的HIV-1亚型趋于多样化。
Objective To determine the subtype of HIV-1 of drug users （DUS） in Guangdong Pearl River delta. Methods HIV-1 pro-viral DNA from buffy coat of 43 DUS in Guangdong was amplified by nested PCR.The C2-V3 regions of HIV-1 ENV gene was sequenced directly from the PCR product and analyzed. Results The 43 DUS were confirmed to be infected with four HIV-1 subtype or Circulating Recombinant Form（CRFs） ：07-BC（ n = 29）,AE（n =9）,08-BC（ n = 3）and B（ n = 2）. Genetic distances showed that the AE group was the closest to CM240 strain isolated in Thailand which is mainly circulating in sexually transmitted infector.The 07-BC group was the closest to C54A strain isolated in Northeastern China. The 08-BC group was the closest to 97CNGX-9F strain isolated in Guangxi,China. The B strain was the closest to r142 strain isolated in Thailand. Conclusion HIV-1 CRFs 07-BC predominates in DUS in Guangdong Pearl River delta.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2006年第3期223-225,共3页
Chinese Journal of Experimental and Clinical Virology
基金
广东省医学科学技术研究基金(WSTJJ2001122644010-5197202120061)
