摘要
目的了解河北省男男同性传播和异性传播人群HIV-1亚型分布和特征。方法从感染者的血浆样品中提取病毒RNA,逆转录后采用套式PCR扩增HIV-1 gag和env基因的部分片段,对PCR产物直接进行核苷酸序列测定,所获序列与各亚型国际参考株序列比对,确定基因型并进行系统进化树分析。结果同性传播的52例HIV-1感染者中,发现了3种HIV-1亚型和重组型,以CRF01-AE为主,占65.4%(34/52),其次为B亚型占30.8%(16/52);异性传播的60例HIV-1感染者中,存在6种亚型和重组型,以B亚型为主占43.3%(26/60),其次是CRF01-AE占30.0%(18/60),CRF07-BC占15%(9/60);两类人群的亚型分布差异有统计学意义(P<0.001)。异性感染人群的各亚型组内基因距离均大于同性感染人群。结论在河北省同性传播人群和异性传播人群中HIV-1亚型分布不均衡。
Objective To study the subtypes distribution and characteristics of HIV-1 strains among men who have sex with men（MSM） and heterosexual in Hebei Province.Methods Viral RNA was extracted from plasma samples,HIV-1 genes（env and gag） were amplified by RT and nested-PCR using specific primer pairs and sequenced directly.The acquired sequences were compared with international subtypes references and their phylogenetic-trees were analyzed to determine the subtype.Results Among 52 MSM of HIV-1 antibody positive,there were 3 kinds of HIV-1 subtypes and re-combinants,of which 65.4%（34/52） cases of CRF01-AE,30.8%（16/52） cases of B.Among 60 heterosexual of HIV-1 antibody positive,there were 6 kinds of HIV-1 subtypes and re-combinants,of which 43.3%（26/60） cases of B,30.0%（18/60）cases of CRF01-AE,15.0%（9/60） cases of CRF07-BC.Comparing by distribution of HIV-1 subtypes,statistical significant difference（P0.001） was found between MSM and heterosexual.The inner groups genetic distance of HIV-1 subtypes were greater in MSM than in heterosexual.Conclusions There are disproportion in the subtypes distribution between MSM and heterosexual in Hebei Province.
出处
《中华疾病控制杂志》
CAS
2011年第12期1035-1037,共3页
Chinese Journal of Disease Control & Prevention
基金
河北省科学技术研究与发展计划(09276102D-2)
