摘要
介绍了用反相高效液相色谱(RP HPLC)测定果汁中有机酸的方法。应用反相C18色谱柱,5g·L-1磷酸二氢铵(pH3.0)溶液作为流动相,流速为1.0ml·min-1,检测波长为210nm,能将果汁中的8种有机酸一次分离测定,重现性好,回收率为94.0%～115.5%。
Eight organic acids (i.e. oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, fumaric acid and succinic acid) were separated on a reversed-phase C_(18) column with 5g·L^(-1) NH_4H_2PO_4 solution (pH 3.0) as the mobile phase, at the flow-rate of 1.0ml·min^(-1). An UV detector (at 210nm) was used in the determination. RSD′s (n=6) for the eight organic acids were found to be 1.0% to 1.9% and average recoveries were in the range of 94.0% to 115.5%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2004年第6期331-333,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
高效液相色谱法
有机酸
果汁
Reversed-phase HPLC
Organic acids
Fruit juice
