摘要
目的 研究 β 七叶皂苷对大鼠脑缺血 再灌注损伤的保护作用。 方法 线栓法制备大鼠大脑中动脉阻断(middlecerebralarteryocclusion ,MCAO)短暂局灶性脑缺血模型 ,缺血前给予 β 七叶皂苷钠 1 5 ,30 ,6 0mg·kg- 1 ,po ,7d ,末次给药 1h后制备MCAO模型 ,缺血 2h ,再灌注 2 4h ,评价神经功能状态、脑水肿和脑梗死范围 ,测定大脑缺血区及非缺血区皮层及海马中SOD ,GSH Px ,CAT和Na+ K+ ATPase的活性及MDA的含量。结果 β 七叶皂苷钠能显著改善脑缺血 再灌注后脑梗死体积 ,减轻脑水肿 ,改善神经功能症状 ,与模型组比较 ,具有显著性差异 (P <0 0 1 ) ;同时 β 七叶皂苷钠组的大鼠MCAO再灌注后 ,其脑内SOD ,GSH Px及ATPase的活性明显高于模型组 ,而MDA的含量明显低于模型组 ,均具显著性差异 (P <0 0 1或P <0 0 5 )。结论 β 七叶皂苷钠对大鼠局灶性脑缺血 再灌注损伤具有明显保护作用。
Aim To investigate the protective effects of sodium β aescin on cerebral ischemia reperfusion injury in rats. Methods Rats were pretreated with sodium β aesein for 7 d and then subjected to cerebral ischemia reperfusion injury induced by a middle cerebral artery occlusion (MCAO). The neurological outcome was evaluated by the Longa′s method; The infarct volume was assessed by hemmatoxylin Eosin staining and the cerebral water content was measured by dry weight method. The activities of SOD, GSH Px, CAT, Na + K + ATPase and the MDA content were measured in the cortex and hippocampus of ischemic and non ischemic hemisphere. Results Sodium β aescin significantly reduced the volume of cerebral infarct and water content, and ameliorated the neurological deficit ( P <0 05). In vehicle treated rats, the activities of SOD, GSH Px and Na + K + ATPase in the cortex and hippocampus of ischemic hemisphere were all decreased ( P <0 01), while the CAT activity was slightly elevated and the MDA of content was significantly increased ( P <0 01) compared with the sham operated group. After treated with sodium β aescin, the effects on recovery of SOD, GSH Px, Na + K + ATPase activities were observed ( P <0 05), and the MDA content was reduced ( P <0 05). Conclusion These results showed that pretreatment with sodium β aescin can attenuate brain injury and its antioxidant activity on rats which encountered cerebral ischemia reperfusion.
出处
《药学学报》
CAS
CSCD
北大核心
2004年第6期419-423,共5页
Acta Pharmaceutica Sinica
