摘要
将210名妊娠3～8wk妇女静脉混合血清经DE_(52)、ConA—Sepharose CL-4B和Sephadex G-100 3次柱层析后获得的微量纯化的具有早孕因子(EPF)活性的孕G-Ⅳ组分,采用微量抗原免疫法免疫小鼠制备鼠抗人EPF免疫血清。间接ELISA测其抗体效价在1:800以上,且为有效阳性。花抑实验亦证明,所制备的免疫血清能中和孕血清及其提取物中EPF活性(与阴性对照比,P>0.05)。该实验方法简便、抗原用量少,适用于微量纯化抗原抗血清及(或)单克隆抗体的制备。
The mice antiserum to human trace early pregnancy factor (EPF) is prepared by pregnant women after 3～8 weeks of gestion. The titer of EPF antibody is more than 1: 800 by indirect ELISA. There are no reactions between the antiserum and other pregnant proteins as hCG,AFP and so on. EPF activity of pregnancy serum and its extracts is neuralized by the prepared antiserum. The results above show that this immune method is simple and practical.
出处
《安徽医科大学学报》
CAS
1993年第2期109-111,共3页
Acta Universitatis Medicinalis Anhui
