摘要
交链孢醇单甲醚(AME)是直接致突变物,可能与食管癌病因有一定关系。氯化血红素(Hemin)是人体内源性成分,本文采用V_(79)细胞6-疏基鸟嘌呤抗性突变试验的方法,观察了Hemin对AME致突变性的影响。结果表明,所用剂量范围内的Hemom对V_(79)细胞无明显毒性和致突变性(P>0.05)。一 定剂量Hemin(15μM)对不同剂量AME (25-100μg/ml)诱发的V_(79)细胞突变频率低于AME单独作用(P<0.01)。此外,与仅含AME对照相比,不同剂量Hemin(5～20μM)能分别降低AME的突变频率(P<0.01),并随Hemin剂量增大,相对抑制率呈增高趋势具有剂量依赖性,显示Hemin对AME致突变性有抑制作用。
Alternariol monomethyl ether (AME) is a direct mutagen, it seems to closely related to the etiology of esophageal cancer. Hemin, an endogeous agent in human body, is a biological pyrrol pigment. In this paper, the effect of hemin on the mutagenicity of AME was investigated with 6-thioguanine resistance (6-TGr) mutagenesis assay in Chinese hamster V79 cells. The results showed that hemin itself had no cytotox-ic effect on Chinese V79 cells, and didn't induce mutations over a range of concentration (5-20μM) (P>0.05). Mutation frequencies induced by AME at the different doses in the presence of 15μM hemin were lower than that of AME alone (P<0.01) . Compared with the control AME(75μg<ml), mutagenicity of AME were remarkably decrea sed by the addition of 5,10, 15 and 20μM hemin , rhspectively (P<0.01). The inhibitory percentage of hemin became greater with the increased dose of hemin. It was demonstrated that the hemin reveals a definite inhibitory effect on the mutagenicity of AME. ,
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第2期101-103,126,共4页
Chinese Journal of Cancer
