摘要
交链孢醇单甲醚(AME)是直接致突变物,可能是林县食管癌病因之一。维胺酸是国内合成的毒性较小的新维甲衍生物,本文采用V_(79)细胞6-疏基鸟嘌呤抗性突变试验的方法,研究了维胺酸对AME致突变性的影响。结果表明,所用剂量范围内的维胺酸对V_(79)细胞无明显毒性和致突变性(P>0.05)。一定剂量维胺酸(15μg/ml)对不同剂量(25～100μg/ml)AME诱发的V_(79)细胞突变频率低于AME单独作用(P<0.01)。此外,与仅含AME对照相比,不同剂量维胺酸(5～20μg/ml)能分别降低AME的突变频率(P<0.05或P<0.01),并随维胺酸剂量增大,相对抑制率增高,呈现剂量相关性,显示维胺酸对AME致突变性有抑制作用,为林县食管癌的化学预防提供了实验依据。
It was known that alternariol monomethyl ether (AME) was a direct mutagen. AME may be one of the etiological factors of esophageal cancer in Linxian County; N-(4-carboxyphenyl) retinamide (RA Ⅱ) is one of the new retinoid derivative ssynthesized in China, which has less toxicity. In this paper, the effect of RA Ⅱ on the mutagenicity of AME was investigated using 6-thioguanine resistance (6-TGr) mutagenesis assay in Chinese hamster V_(79) cells; The experimental results showed that RA had neither cytotoxicity nor mutagenicity on V_(79) cells within the concentration range from 5μg/ml to 20μg/ml (P>0.05). Mutation frequencies induced by AME at different dosage in the presence of 15μg/ml RA Ⅱ were lower than that of AME alone (P< 0.01). Compared with the control group (AME 75μg/ml), mutagenicity of AME were effectively reduced by addition of RA Ⅱ from 5 to 20μg/ml (P<0.05 or P<0.01).The inhibitory effect on RA Ⅱ became greater with increasing dosage, in a dose dependent manner. The inhibition on the mutagenicity of AME by RA Ⅱ provided a good experimental evidanee for chemoprevention of esophageal cancer in Linxin County a high incidence area of esophageal cancer.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1991年第6期617-621,共5页
Chinese Journal of Pathophysiology
关键词
食管肿瘤
维胺酸
链格孢属
突变
Esophageal neoplasms
Alternatia
Retinoids
mutation
