摘要
本文应用碱性洗脱-荧光分析法对互隔交链孢酶产生的两种主要毒素——交链孢酚单甲醚(AME)和交链孢酚(AOH)引起人胚肺2BS细胞DNA单链断裂效应进行了探讨。实验结果表明,AME和AOH都能导致2BS细胞DNA单链断裂,并有剂量依赖性,与溶剂对照相比,DNA在滤膜上的存留比明显降低,如洗脱6小时对照组的存留比为0.87±0.02,AME浓度为10,25,50,100μg/ml时分别为0.57±0.04、0.45±0.02、0.30±0.02、0.18±0.01,AOH浓度为0.1、1、5、10μg/ml时分别为0.68±0.03、0.54±0.01、0.47±0.03、0.34±0.01,统计学分析均有显著意义(P<0.01)。本实验还用同样的方法检测了维胺酸Ⅲ号(RⅢ)对AME和AOH所致的DNA单链断裂的抑制作用。
The alkaline elution and fluorometrie DNA assay were adapted to the evaluation of DNA single-strand breaks in the cultured human fetal lung 2BS cell. the DNA damage was induced in vitro by the treatment with Alternariol monomethyl ether (AME) and Alternariol (AOH) which were the main mycotoxins of Alternariol alternata. The results showed that both AME and AOH could induce the DNA single-strand breaks in the cultured human fetal lung 2BS cells and there existed the dose-response association betweenthe fractions of DNA remaining on the filters and the doses. The fractions of DNA remaining on the filters were 0.57±0.04, 0.45±0.02, 0.30±0.02, 0.18±0.01 respetively under the AME concentration of 10, 25, 50, 100μg/ml and were 0.68±0.3, 0.54±0.01, 0.47±0.03, 0.34±0.01 respetively under the AOH concentration of 0.1, 1, 5, 10μg/ml when DNA was eluted for 6hr. It were significantly different from the fractions by solvent control (0.87±0.02) (P<0.01). The same method was also used to test the inhibitory effects of R Ⅲ on the DNA single-strand breaks induced by AME and AOH.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1991年第4期396-399,共4页
Chinese Journal of Pathophysiology
关键词
链格孢属
毒素
食管肿瘤
维甲酸
Alternaria
Mycotoxins
Retinoids
DNA, single-strand
Esophageal neoplasms
