摘要
检查API2-MALT1融合基因多种变异体mRNA在MALT淋巴瘤中的表达情况,探讨其表达与MALT淋巴瘤的临床病理特征及预后的关系。方法:收集胃和肺MALT淋巴瘤石蜡包埋标本共29例,慢性淋巴结炎5例。采用RT-PCR和巢式PCR相结合检测MALT淋巴瘤中API2-MALT1的mRNA。将29例分为API2-MALT1mRNA表达组及未表达组,了解两组患者临床病理学特征和预后的差异。结果:MALT淋巴瘤中两种API2-MALT1变异体被检出,总检出率为48.2%,慢性淋巴结炎未检出,与未表达组相比,表达组临床病理分期偏低,细胞增殖活性和淋巴结受累有降低和减轻的趋势,两组间病变范围、浸润深度等特征及预后无明显差异。结论:API2-MALT1mRNA的表达可能与MALT淋巴瘤的淋巴结受累、临床病理分期及细胞增殖有关,与MALT淋巴瘤的浸润深度、病变范围及预后等无明显相关;不同的变异体表达与MALT淋巴瘤的发生部位有关。
Objective: To investigate the expression of various variants of API2- MALT1 fusion gene in MALT lymphoma and its effects on the clinicopathological feature and prognosis of MALT lymphoma. Methods: Twenty-nine archival cases of gastric and pulmonary MALT lymphoma were selected and reviewed. Five cases of chronic lymphoadenitis were used as negative control. Four kinds of variant of API2-MALT1 fusion gene and β-actin mRNA(internal control) were assayed in all samples by RT-PCR and nested PCR. Twenty-nine samples were divided into two groups in terms of the expression of API2-MALT1 mRNA or not. The relationship between the expression and the clinicopathological feature and prognosis of MALT lymphoma was investigated by the stains of HE, IHC and follow-up. Results: β-actin was detected in all samples. Two variants of the fusion gene were found in 14 of 29(48.2%) cases with MALT lymphoma(gastric MALT lymphoma:9/19, 1446/1123 predominantly; pulmonary MALT lymphoma: 5/10, 1446/814, predominantly), and none of chronic lymphoadenitis. Comparing with those without API2-MALT1 mRNA,the staging(P=0.134), cellular proliferation(P=0.0870) and involvement of lymph node(P=0.0541) tended to be lower and milder in the group with API2-MALT1 fusion transcript, there were no distinct difference in the extent of lesion, the depth of invading and prognosis between two groups. Conclusion: The expression of API2-MALT1 mRNA may be associated to the staging, involvement of lymph node and cellular proliferation of MALToma, but not to the extent of lesion, depth of invading and prognosis of MALToma. The expression of the different variants are correlated with the site of MALT lymphoma.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第10期550-553,共4页
Chinese Journal of Clinical Oncology
基金
美国中华医学基金资助(CMB00-722)
