摘要
目的研究从石蜡包埋组织中提取RNA检测黏膜相关淋巴组织(MALT)淋巴瘤T(11,18)易位所致API2-MALT1(凋亡抑制蛋白2-黏膜相关淋巴瘤转位基因1)融合基因。方法采用酸性酚氯仿法从石蜡包埋组织中提取RNA,通过逆转录聚合酶链反应(RT-PCR)扩增API2-MALT1融合基因,并通过转染测序检测基因。结果全部病例均检测到葡糖6磷酸脱氢酶(G6PD)重排;101例MALT淋巴瘤中API2-MALT1融合基因阳性率为21.8%,其中低度恶性的阳性率为25.0%,转化型的阳性率为13.8%;80例弥漫大B细胞淋巴瘤仅1例阳性;DNA测序结果与国际报道的序列相符。结论石蜡包埋组织中提取RNA方法稳定,可为疾病相关基因检测提供有效手段,在科研和临床方面有广阔的应用前景。
Objective To extract RNA from formalin-fixed and paraftin-embedded tissues of mucosa-associated lymphoid tissue lymphoma and detect API2-MALT1 gene of chromosome translocation t （11;18）（q21;q21）. Methods RNA was extracted from tissues using paraffin block RNA Isolation kit according to the manufacture＇s protocol, and then examined by reverse transcription-polymerase chain reaction （RT-PCR） and proved by sequencing. Results G6PD were detected in all samples; API2-MALT1 gene was 21.8% positive in MALT lymphoma, among them 25.0% positive samples were in low grade and 13.8% positive samples were in the transformed type. Only 1 positive in 80 cases of diffuse large B cell lymphoma. And the result of sequencing was consistent with the International sequence. Conclusions The way to extract RNA from paraffin-embedded tissue is stable and feasible, and it is a good method for disease-associated gene detection.
出处
《北京医学》
CAS
2006年第3期129-131,共3页
Beijing Medical Journal
基金
国家自然科学基金
海外青年学者基金资助项目(编号30428018)
