摘要
采用微波辅助方法提取了酵母细胞中的谷胱甘肽。研究表明 ,在 65 0W功率下对酵母泥直接微波处理 1 2s ,再以 2 0mL/g的物料比提取 1 0min ,或者物料比为 1 5mL/g的酵母悬浮液在 65 0W下处理 60s,都能有效地提取谷胱甘肽 ,提取率分别达到 2 0 89mg/g和 2 1 1 7mg/g。和其他提取方法比较 ,微波辅助提取是一种节省能源、快速、简便、有效的方法。
Glutathione,one of the most important biological active substance, plays a central role in metabolic pathways of live organisms MAE, a new method, is reported here Both method a and b can extract GSH effectively In method a, moisture yeast cell was treated with microwave under 650?W for 12?s, then extracted with 20?mL/g water for 10?min In method b, suspended liquid (15?mL/g) treated with microwave under 650 W for 60 s The extract rates of glutathione were 20 89 mg/g and 21 17?mg/g respectively Compared with other methods, MAE was proved to be energy saving, fast, brief and effective method for the extraction of glutathione
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2004年第4期27-31,共5页
Food and Fermentation Industries
基金
江苏省高校高新技术产业发展项目 (No.JH0 2 - 1 0 1 )
关键词
酵母细胞
谷胱甘肽
微波辅助提取
微波处理
物料比
glutathione, microwave assisted extraction (MAE), Candida utilis, extraction
