摘要
利用生物技术方法生产谷胱甘肽（ＧＳＨ）的研究正方兴未艾。本研究室已对面包酵母生产ＧＳＨ进行了较为深入的研究［１，２］，但距工业化生产尚有一段距离，关键在于高ＧＳＨ含量面包酵母的选育较为困难。近来这一方向的研究重点又转向构建重组大肠杆菌来生产ＧＳＨ。虽...
The effect of initial glucose concentration on the production of GSH by recombinant E.coli in shaking flask culture were investigated and the fermentation process was analyzed thoroughly. Obviously inhibition occured when the initial glucose concentration exceeded 20g/L -1 , suitable glucose feeding time was 8 hours. The effect of three kinds of feeding strategies on the production of GSH in 2L jar fermenter were compared, exponential feeding method was confirmed to have remarkable predominance in both enhancing cell density, productivity and promoting the yield of cell to glucose. The cell density, total GSH and productivity reached 80g/L -1 , 880mg·L -1 and 3.2g/(L·h) -1 at 25h by using exponential glucose feeding strategy, respectively.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第4期452-455,共4页
Chinese Journal of Biotechnology
基金
霍英东高等院校青年教师基金
江苏省跨世纪学科带头人科研基金
关键词
谷胱甘肽
大肠杆菌
高密度培养
培养模式
Glutathione, recombinant Escherichia coli , high cell density culture
