摘要
建立高效液相色谱检测发酵液中木糖和木糖醇含量的分析方法。色谱柱为HypersilNH2 柱 (4 .6mmi.d.× 2 5 0mm ,5 μm) ,柱温 3 5℃ ,流动相为乙腈—水 (80∶2 0 ) ,流速 1 .0mL .min 1,示差折光检测器检测。木糖和木糖醇在 3 .0～ 60mg.mL 1范围内 ,峰面积与其浓度线性关系良好 (г=0 .9995 ) ;平均回收率分别为 96.0 7% (n =5 ,RSD =0 .5 1 % )和 97.47% (n =5 ,RSD =1 .1 3 % )。方法简便、快速、准确。
A method for the determination of xylose and xylitol in fermentation broth by high performance liquid chromatography is described. The operating conditions were Hypersil NH_2 column(4.6 mm i.d.×250 mm,5 μm)at 35 ℃ and differential refractive index detector. Mobile phase was acetonitrile-water(80∶20;volume ratio)and the flow-rate was 1.0 mL.min^(-1). The linear range was 3.0 mg.mL^(-1) to 60.0 mg.mL^(-1),the correlation coefficient was 0.999 5. The corresponding average recoveries were 96.07%(n=5,RSD=0.51%)and 97.47%(n=5,RSD=1.13%)respectively. The method is simple,rapid and accurate.
出处
《广西植物》
CAS
CSCD
北大核心
2004年第3期275-277,198,共4页
Guihaia
基金
广西自然科学基金资助项目 (98110 15 )
关键词
高效液相色谱法
检测
发酵液
木糖
木糖醇
微生物
xylose
xylitol
fermentation process
high performance liquid chromatography
