摘要
用亲合组织化学技术,以UEA—I,ConA,PNA三种凝集素对30例鼻咽上皮重度不典型增生、化生(癌前病变),70例鼻咽癌及30例正常鼻咽粘膜上皮进行观察对比,了解其分布定位及含量的差异。结果发现:荆豆凝集素(UEA—I)在正常鳞状上皮多呈膜性分布;鼻咽癌癌细胞多呈阴性反应;而鼻咽癌前病变的细胞则显示膜性及胞浆性强阳性反应,与正常上皮及癌巢对比鲜明,UEA—I还能显示间质异常增生的血管,说明它是一种协助诊断鼻咽癌前病变很有希望的一种凝集素。而PNA及ConA则对鼻咽癌前病变的诊断价值不大。
By the use of lectin affinitive histochemical method, the affinity of normal, dysplastic, neoplastic nasopharyngeal epithelium to three kind of lectins (UEA-I, ConA and PNA) were studied. UEA-I (Ulex european Agglutinin-I) displayed membrane distribution in normal squamous epithelium, but most of nasopharyngeal carcinoma cells showed negative reaction to UEA-I Notably, severe dysplastic epithelium (precancerous lesion) exhibited a strong membranous and cytoplasmic affinity, that contrasted sharply with the normal epithelium and carcinoma ceils. The statistkally significant differencein the content and distributed location of lectin UEA-I in these three groups, suggested that UEA-I may be a useful lectin for the diagnosis of precancerous lesion of nasopharynx. But PNA and ConA have no diagnostic Value.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1992年第1期18-20,T000,共4页
Chinese Journal of Cancer
基金
国家教委博士点科研基金
关键词
鼻咽肿瘤
病变
凝集素
诊断
Nasopharyngeal carcinoma Precancerous lesion Lectin-affinitive histochemistry
