摘要
目的 探讨早期应用不同剂量氨基胍对内毒素休克模型兔肾功能的保护作用及氨基胍治疗的剂量、时间依赖性。方法 4 0只新西兰白兔麻醉后被随机分成 5组 :对照组、内毒素组、氨基胍一组、二组、三组。除对照组外 ,其他四组每只兔静脉推注大肠杆菌O5 5B5内毒素 (LPS) 4 0 0 μg/kg ,当平均动脉压下降为原来的 30 %时 ,休克诱导成功。氨基胍一、二、三组每只兔分别静脉注射 30、5 0、10 0mg/kg的氨基胍 ,对照组及内毒素组给予相应剂量的生理盐水。在注射内毒素前 (T0 )、休克时(T)、休克后第 1(T1)、2 (T2 )、3(T3 )、4 (T4)、5 (T5)、6 (T6)小时记录尿量 ,在T、T2 、T4、T6时间点测定血硝酸盐、亚硝酸盐 (NO-3 /NO-2 ,NO的代谢产物 )、尿素氮 (BUN)、肌酐 (Scr)、尿视黄醇结合蛋白(RBP)。结果 LPS可引起血NO-3 /NO-2 、BUN、Scr、尿RBP显著升高 [分别从T点的 (47± 5 ) μmol/L、(5 8± 1 5 )mmol/L、(41± 10 ) μmol/L、(2 4 0± 6 1)ng/L ,升高至T6点的 (16 0± 18) μmol/L、(15 5±1 8)mmol/L、(16 6± 2 3) μmol/L、(15 80± 180 )ng/L ,P均 <0 0 1]。尿量显著减少 [从T0 点的 (17 6± 2 8)ml降低到T6点的 (1 3± 0 6 )ml,P <0 0 1]。三个氨基胍组均可减轻NO-3 /NO-2 、BUN、SCr、RBP升高?
Objectives To explore the effects of aminoguanidine (AG) in different dosages on renal function in endotoxin induced rabbits shock model in the early stage and to approve the effects of dose depended and time depended of AG Methods Fourty New Zealand rabbits under anaesthesia were randomly divided into 5 groups: sham group, LPS group, the first group of AG, the second group of AG, the third group of AG Each rabbits from the four groups received Escherichia Coli O55B5 LPS 400 μg/kg to induce endotoxic shock except sham group Edotoxic shock was diagnosed when the mean arterial pressure (MAP) decreased to 30% Each rabbits in sham group and LPS group received 5ml NS, in the other three groups were infused with AG 30 mg/kg (the first group of AG ), 50mg/kg (the second group of AG), 100 mg/kg (the third group of AG) in 5ml NS, respectively Urine output was recorded at the following time points, before injecting IPS (T 0), shock (T), 1h (T 1), 2h (T 2), 3h (T 3), 4h (T 4), 5h (T 5) and 6h (T 6) after shock Plasma nitrate and nitrite (NO - 3/NO - 2, stable products of NO), BUN, Scr, RBP were determined at the time points of T, T 2, T 4 and T 6 Results LPS increased NO - 3/NO - 2, BUN, Scr, RBP[from (47±5)μmol/L, (5 8±1 5)mmol/L,(41±10) μmol/L, (240±61)ng/L (T 0) to (160±18)μmol/L,(15 5±1 8) mmol/L,(166±23) μmol/L,(1580±180) ng/L (T 6), respectively, P <0 01]; Urine output decreased significantly[from (17 6±2 8)ml(T 0) to (1 3±0 6)ml(T 6), P <0 01] AG attenuates the increasing of NO - 3/NO - 2, BUN, Scr and RBP, and decreasing of urine output NO 3 /NO 2 of the first, second and third group of AG at T 6 were (58±8), (50±14) and (46±9)μmol/L, respectively Compared to LPS group, there was a significant difference( P <0 01) BUN was (8 2±2 9), (7 5±1 9) and (5 5±1 8) mmol/L, respectively at T 6 Compared to LPS group, there was a significant deference( P <0 01) RBP was (350±60), (272±72) and (248±103) ng/L, respectively at T 6 (compared to LPS group, there was a significant deference P <0 05,<0 05,<0 01) Urine output was (11 1±2 4), (12 1±1 3) and (17 1±2 4)ml, respectively on T 6 (compared to LPS group, there was a significant deference, P <0 01) AG of 100 mg/kg showed the best effect among three AG groups Conclusion AG inhibited NO formation in dose depended and time depended way AG attenuated the changes of renal function induced by NO
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2004年第3期206-209,共4页
Chinese Journal of Pediatrics
