摘要
目的 研究腺病毒 1 2型E1B 5 5kD蛋白 (Ad1 2E1B)对hDaxx转录调控的影响作用。方法 利用酵母双杂交法分析hDaxx与Ad1 2E1B的相互作用 ;共免疫沉淀试验和Westernblotting测定两种蛋白质在细胞内外的结合反应 ;通过虫荧光素酶报道基因系统 ,分析hDaxx对其转录活性的影响。结果hDaxx在细胞内外直接与Ad1 2E1B结合 ,并与全序列Ad1 2E1B发生相互作用 ;Ad1 2E1B显著增加hDaxx的转录抑制活性 (P <0 .0 1 )。结论 Ad1 2E1B与hDaxx相互作用并增强hDaxx的转录抑制活性。
Objective To study the transcriptional regulating effect of hDaxx interacting with adenovirus(Ad)12E1B 55 kilodalton protein(Ad12E1B).Methods Interaction of hDaxx and Ad12E1B was analyzed by the yeast two-hybrid assay. The direct binding of hDaxx and Ad12E1B was analyzed using coimmunoprecipitation and Western blotting in vivo and in vitro. The activity of Luciferase report gene, which was regulated by hDaxx modulated TK promoter, was detected in automat luminometer. Results hDaxx interacted with Ad12E1B by its full length. hDaxx bound directly to Ad12E1B in vivo and in vitro. Ad12E1B enhanced the transcriptional repression activity of hDaxx. Conclusion Ad12E1B interacting with hDaxx enhanced transcriptional repression activity of hDaxx.
出处
《南华大学学报（医学版）》
2004年第3期286-289,共4页
Journal of Nanhua University(Medical Edition)
基金
湖南省卫生厅资助项目 (No .B2 0 0 3 -10 1)
湖南省教育厅资助项目 (No .0 2C3 91)
