摘要
为研究单核—巨噬细胞和ECV30 4细胞是否表达死亡相关蛋白以及影响死亡相关蛋白表达的因素。分别用氧化型低密度脂蛋白 (10 0mg L)孵育THP 1细胞 ,H2O2 (1mmol L)孵育ECV30 4细胞 ,用逆转录聚合酶链反应检测死亡相关蛋白mRNA的表达。结果发现 ,THP 1细胞及ECV30 4细胞均表达死亡相关蛋白 ,氧化型低密度脂蛋白和H2O2能分别诱导THP 1细胞和ECV30 4细胞死亡相关蛋白mRNA表达增高。实验结果提示死亡相关蛋白可能参与调节氧化应激诱导的单核—巨噬细胞和内皮细胞的凋亡。
Aim To explore whether monocyte-derived macrophages and ECV304 endothelial cells express death-associated protein (Daxx) and expression induced by oxidative stress. Metheds Reverse transcription polymerase chain reaction (RT-PCR) were performed to detect Daxx mRNA expression in THP-1 monocyte-derived macrophages and ECV304 cells induced by ox-LDL and H 2O 2 respectively. Results THP-1 monocyte-derived macrophages and ECV304 cells expressed Daxx, Daxx mRNA expression increased after THP-1 monocyte-derived macrophages and ECV304 cells were incubated with ox-LDL (100 mg/L) and H 2O 2 (1 mmol/L) respectively. Conclusion Daxx may be involved in apoptosis induced by Oxidative stress in THP-1 monocyte-derived macrophages and ECV304 cells.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第2期162-164,共3页
Chinese Journal of Arteriosclerosis
