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腺病毒E1B55kD癌蛋白与hDaxx的相互作用 被引量：2

Interaction of Adenovirus E1B 55kD Oncoproteins with hDaxx
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摘要 为了探讨腺病毒 (adenovirus,Ad)E1B 5 5kD癌蛋白 (AdE1B 5 5kD)打破hDaxx和PML共定位细胞核的作用机制 ,本文利用体内外共免疫沉淀反应研究AdE1B 5 5kD与hDaxx的结合反应 ,并通过酵母双杂交体系测定两种蛋白质的相互作用及其作用的氨基酸残基序列。结果显示 :Ad2E1B 5 5kD通过C端 5 8个氨基酸 (aa)与hDaxx结合并发生相互作用。Ad12E1B 5 5kD与hDaxx结合需全序列aa及其构象。共免疫沉淀反应和Westernblot结果证实Ad2 / 5或Ad12E1B 5 To question the reason of co localization of hDaxx and PML in PODs disrupted by Adenovirus(Ad) E1B 55 kilodalton oncoproteins(Ad E1B 55kD), the interaction of Ad E1B 55kD with hDaxx was analyzed by the yeast two hybrid assay, and the direct binding of Ad E1B 55kD with hDaxx was studied by coimmunoprecipitation reaction \%in vivo\% or \%in vitro\%. The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C terminus 58 amino acids of Ad2 E1B 55kD or the full length of Ad12 E1B 55kD. Ad2/5 or Ad12 E1B 55kD directly binds hDaxx \%in vivo\% or \%in vitro.\%
作者 万艳平 吴移谋 谭立志 廖端芳
机构地区 南华大学基础医学院微生物学教研室 南华大学基础医学院药理学教研室
出处 《中国病毒学》 CSCD 2002年第3期230-233,共4页 Virologica Sinica
基金 国家教育部高等学校骨干教师资助计划资助 (2 0 0 0 65 )
关键词 腺病毒 E1B55KD癌蛋白 HDAXX 相互作用 Ad E1B oncoproteins hDaxx Yeast two hybrid assay Interaction
分类号 R730.2 [医药卫生—肿瘤]
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参考文献2

二级参考文献13

  • 1[1]Yang, X., L., Khosravi-Far, R., Chang, H., Y., et al. Daxx, a novel Fas-binding protein that activates JNK and apopotosis[J].Cell, 1997,89:1067 ～ 1076.
  • 2[2]Ishov, M., A., Sotnikov, G., A., Negorev, D., et al. PML is critical for ND10 formation and recruits the PML-interacting protein Daxx to this nuclear structure when modified by SUMO-1 [J].J. Cell Biology, 1999,147: 221 ～ 233.
  • 3[3]Pluta, A.,F., Earnshaw, W., C. & Goldberg, I., G. Interphase specific association of intrinsic centromere protein CENP-C with Hdaxx, a death domain-binding protein implicated in Fas-mediated cell death[ J ]. J. Cell Science, 1998,111: 2029 ～ 2041.
  • 4[4]Liu, y., Colosimo, A.L., Yang, X.J., et al. Adenovirus E1B 55-kilodalton oncoprotein inhibits p53 acetylation by PCAF[J].Mol. & Cell. Bio.2000,20:5540～5553.
  • 5[5]Sternsdorf,T.,Grotzinger,T.,Jensen,K.,et al. Nuclear dots: actors on many stages[J]. Immunobiology, 1997,198: 307 ～ 331.
  • 6[6]Kaelin, W. G. The emerging p53 gene family J [J]. Natl Cancer Inst, 1999,91:594～598.
  • 7[7]Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., et al.Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region[J ]. DNA and Cell Bio, 1997,16:1289～ 1298.
  • 8[8]Li, H., Leo, C., Zhu, J., et al. Sequestration and inhibition of Daxx-mediated transcriptional repression by PML [J]. Molecular and cellular biology, 2000,20:1784～ 1796.
  • 9[1]Wienzek, S., Roth, J. and Dobbelstein, M. E1B 55-kilodalton oncoproteins of adenovirus types 5 and 12 inactivate and relocalize p53, but not p51 or p73, and cooperate with EAorf6 proteins to destabilize p53[J]. J. Virol., 2000,74:193～202.
  • 10[2]Punga, T. and Akusjarvi, G. The adenovirus-2 E1B-55K protein interacts with a mSin3A/histone deacetylase 1 complex[J]. FEBS Letters, 2000,476:248～252.

共引文献11

同被引文献21

  • 1Yang X, Khosravi FR, Chang HY, et al. Daxx, a novel Fas-binding protein that activates JNK and apoptosis[J]. Cell, 1997, 89(7): 1067-1076.
  • 2Pluta AF, Earnshaw WC, Goldberg IG. Interphase-specific association of intrinsic centromere protein CENP-C with hDaxx, a death domain-binding protein implicated in Fas-mediated cell death[J]. Cell Sci, 1998, 111(14) : 2029-2041.
  • 3Ko YG, Kang YS, Park H, et al. Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm[J]. J Biol Chem, 2001, 276(42): 39103-39106.
  • 4Charette SJ, Lavoie JN, Lambert H, et al. Inhibition of Daxx-mediated apoptosis by heat shock protein 27[J]. Mol Cell Biol, 2000, 20(20): 7602-7612.
  • 5Muromoto R, Yamamoto T, Yumioka T, et al. Daxx enhances Fas-mediated apoptosis in a murine pro-B cell line, BAF3[J]. FEBS Lett, 2003, 540(1-3): 223-228.
  • 6Shinoda S, Skradski SL, Araki T, et al. Formation of a tumour necrosis factor receptor 1 molecular scaffolding complex and activation of apoptosis signal-regulating kinase 1 during seizure-induced neuronal death[J] . Eur J Neurosci, 2003, 17(10): 2065-2076.
  • 7Zhong S, Salomoni P, Ronchetti S, et al. Promyelocytic Leukemia Protein (PML) and Daxx participate in a novel nuclear pathway for apoptosis[J]. J Exp Med, 2000, 191(4): 631-640.
  • 8Michaelson JS, Bader D, Kuo F, et al. Loss of Daxx, a promiscuously interacting protein, results in extensive apoptosis in early mouse development[J]. Genes Dev, 1999, 13(15): 1918-1923.
  • 9Liu Y, Colosimo AL, Yang XJ, et al. Adenovirus E1B 55-kilodalton oncoprotein inhibits p53 acetylation by PCAF[J]. Mol Cell Biol, 2000, 20(15): 5540-5553.
  • 10Zhao LY, Colosimo AL, Liu Y, et al. Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and eliminates enhancement of p53-dependent transcription by Daxx[J]. Virology, 2003, 77(21): 11809-11821.

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中国病毒学
2002年 第3期

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