摘要
利用葡萄饮料厂生产的废料葡萄皮提取粗蛋白,并对影响粗蛋白提取率的几个因素进行了研究。这些因素包括提取溶剂的选择、提取剂浓度、提取温度、提取时间等,并设计正交实验选取最佳提取条件,即:以NaOH溶液为提取剂,NaOH浓度为0.8 mol·L-1,提取温度70℃,提取时间为80min。提取得到的粗蛋白中蛋白质质量分数为71.38％。
Crude proteins were extracted from waste grape skin obtained from grape drink factory. Some influence factors on extraction ratio of crude proteins were discussed. These factors were choice of extraction solvent, concentration of solvent, temperature and time of extraction, etc. The optimum conditions of the best extraction ratio was tested by orthogonal experiment. And the results were as follows: sodium hydroxide was optimum extraction solvent and concentration used at 0. 8mol/L, extracting solution was heated for 80 min at 70℃.
