摘要
脑脊液髓鞘碱性蛋白(MBP)自身抗体固相发光免疫分析法,是一项免疫检测新技术,目前国外尚未见到对脱髓病研究的报道。本文作者于1988.5～1989.3首先采用本法并证实其可靠性后,进入临床研究,对36例脱髓鞘病及20例其他CNS疾病者的对照组,用三项免疫指标行对比分析。CSFMBP自身抗体含量及其和同量IgG自身抗体的百分比值分别为69.23±11.95ng/ml及3.24±0.52ug/mg±gG和对照组相比,P值分别为<0.05及<0.001,有显著差异,对本病诊断有意义。
Luminescence immunoassay of CSF MBP autoantibodies in Demylinating disease is a new technique, and haven't ever been reported in the past international literature from 1988.5—1990.3, we developed an assay method for autoantibodies angainst CSF MBP by coating solid phase with MBP and added luminescence substance Lumino to labelling sheep anti human IgG with ABEI. The contents of IgG and autoantibodies against MBP in CSF were calculated from the same standard curve of IgG. The results showed that CSF MBP autoantibodies contents in 36 Demylinating patients and 20 other CNS patients were 3.24±0.52 and 0.72 ± 0.16μg/mgIgG respectively (P<0.001). We suggest that this parameter is much more accurate than that of IgG for the differential diagnosis and determine the activity of Demylinating from other CNS diseases. The mechanism was also discussed in this paper.
出处
《军医进修学院学报》
CAS
1992年第1期16-19,共4页
Academic Journal of Pla Postgraduate Medical School
关键词
自身抗体
免疫法
脱髓鞘疾病
MBP
Cerebrospinal fluid proteins Autoantibodies Immunization Demyelinatin diseasesg
