摘要
目的 探讨血管内皮生长因子 (VEGF)在增生性血管瘤组织、邻近组织和外周血中的表达水平和分布规律 ,进一步了解血管瘤的发生机制。方法 取增生性血管瘤患儿外周血清和术中瘤组织及其邻近组织标本 ,以相同年龄非相关患儿作对照 ,用酶联免疫法测定血清VEGF水平 ;对肿瘤组织及其相邻组织行cDNA合成和PCR扩增 ,提取VEGFmRNA ,并进行定量分析。结果 血管瘤患儿血清VEGF水平明显高于对照组 (P <0 .0 5 ) ;血管瘤组织的VEGFmRNA电泳阳性率为 10 0 % (9/ 9) ,邻近组织为 88.9% (8/ 9) ,对照组为 11.1% (1/9) ,经检验血管瘤及其邻近组织与对照组有显著性差异 (P均 <0 .0 1)。荧光定量测定mRNA在血管瘤及其邻近组织无显著差异 (P <0 .0 5 ) ,而两者与对照组均有显著差异 (P均 <0 .0 1)。结论 增生性血管瘤组织及患儿外周血VEGF明显增高 ,对血管内皮细胞增生和分化成血管瘤细胞有促进作用 。
Objective To investigate the expressons of vascular endothelial growth factor(VEGF) in childhood proliferated hemangiomas ,its adjacent tissue and serum for further understanding the biochemical characteristics of hemangiomas.Methods The blood sample and proliferated fresh tissues from hemangiomas and its adjacent tissues were collected. The VEGF concentration was measured by ELISA test. The cDNA of VEGF composed first and the quantity of VEGF mRNA from the tumor and its adjacent tissues were detected and measured in the fluroscent PCR machine by reverse transcription-polymerase chain reaction(RT-PCR). Results VEGF in patient's serum was significantly higher than that of control group. The electrophoresis showed strong positiveness,the expressions of VEGF were 100 %(9/9) and 88.9 %(8/9) in hemangiomas and its adjacent tissues respectively. The high expression of VEGF was detected and quantified by the fluroscent PCR machine. The amount of VEGF mRNA in hemangiomas and its adjacent tissues were much higher than those of control group(P<0.01). However there was no difference between the tumor and its adjacent tissues.Conclusion VEGF may stimulate vascular endothelial cells to proliferate and differentiate toward the hemangiomas. The measurement of VEGF in the peripheral blood might be a useful method for analying hemangiomas classification and recurrence evaluation.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2004年第5期378-379,403,共3页
Journal of Applied Clinical Pediatrics
基金
黑龙江省自然科学基金资助项目 (D991 0 )
关键词
血管内皮生长因子
血管瘤
细胞增生
儿童
vascular endothelial growth factor
hemangioma
cell proliferation
children
