摘要
目的观察人血管瘤裸小鼠移植模型超微结构动态变化。方法将手术切取的增生期婴幼儿血管瘤组织移植到裸小鼠皮下,于移植后d2、d5、d15、d30、d45及第2、3、4、6个月切取移植瘤。戊二醛固定后行透射电镜观察。结果移植后早期内皮细胞大量变性、坏死。d30细胞出现增生表现,内皮细胞数量增加,部分细胞体积增大,核浆比增大,核质淡染。2个月时内皮细胞密集,部分内皮细胞变扁平。此后内皮细胞数量减少,出现胞质脱落、胞质固缩、次级溶酶体形成、染色体边集、核碎裂,伴基质中脂肪和胶原沉积。结论婴幼儿血管瘤组织移植到裸小鼠体内,经过缺血期后,瘤性内皮细胞可快速增生,继而缓慢消退,其超微结构变化与人血管瘤自然演变过程相似。
Objective To investigate the development of ultrastmcture in grafts from a xenograft model of human hemangioma on nude mice. Methods Human infantile hemangioma tissue was obtained in surgery and planted into subcutaneous layer of nude mice in small pieces. The grafts were harvested on the 2^nd, 5^th, 15^th, 30^th, 45^th day and then at 2^nd, 3^rd, 4^th, 6^th month for transmission electron microscopic view. Results In early stage after transplantation, signs of degeneration and necrosis were more evident. On the 30th day, the number of endothelial cells increased and some of the endothelial cells were larger in size with high nuclear to plasm ratio. At the 2^nd month, the number of endothelial cells increased, while some of these cells turned flat. Later, high endothelial cells decreased in number and collapse and pyknosis of plasm, formation of secondary lysosome, margination of chromatin, fragmentation of nucleus were evident with surrounding deposition of adipose and collagen. Conclusions With xenotransplantation of human infantile hemangioma tissue onto hude mice, the grafts may survive ischemia and proliferate, and then gradually involute： The development resembles a natural course of human infantile hemangioma.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第11期652-654,共3页
Journal of Applied Clinical Pediatrics
基金
教育部高等学校博士学科点专项科研基金项目资助(20020610068)
关键词
血管瘤
模型
动物
小鼠
裸
内皮细胞
超微结构
hemangioma
models, animal
mice, nude
endothelial cells
ultrastructure
